Abbreviation
Recombinant Human TNFSF13B protein, partial, Biotinylated (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized human BCMA (CSB-MP023974HU1) at 5 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.1752-0.3657 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C (CSB-MP853495HU) at 2 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.2699-0.5613 ng/ml.
Alternative Names
B lymphocyte stimulator （BLyS） （B-cell-activating factor） （BAFF） （Dendritic cell-derived TNF-like molecule） （TNF- and APOL-related leukocyte expressed ligand 1） （TALL-1）
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
134-285aa
Target Protein Sequence
AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal hFc-Avi-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized human BCMA (CSB-MP023974HU1) at 5 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.1752-0.3657 ng/ml.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C (CSB-MP853495HU) at 2 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.2699-0.5613 ng/ml.
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The purity of TNFSF13B was greater than 90% as determined by SEC-HPLC.
Description
TNFSF13B (BAFF) is a critical B-cell survival factor whose interactions with BCMA and BAFF-R drive proliferation, differentiation, and antibody class-switching, making it a key target in autoimmune and oncology research. This biotinylated recombinant human TNFSF13B (aa 134–285) carries an N-terminal hFc-Avi tag and demonstrates sub-nanogram binding potency, with EC50 values of 0.18–0.37 ng/ml against immobilized BCMA and 0.27–0.56 ng/ml against TNFRSF13C (BAFF-R) in functional ELISA. These binding characteristics support use as a detection reagent or coating antigen in antibody development campaigns, as a positive control in BAFF-quantification assays, and as a tool for characterizing NF-κB pathway activation downstream of BAFF-R engagement. Produced in mammalian cells with purity exceeding 90% and endotoxin levels below 1.0 EU/μg, this protein satisfies the quality criteria typical for immune cell-based assays where LPS-driven artifacts must be excluded.
