Abbreviation
Recombinant Human TGF-β3 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
≤10 EU/mg by the LAL method
Activity
Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2ng/mL.
Alternative Names
Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP
Species
Homo sapiens (Human)
Expression Region
301-412aa
Target Protein Sequence
ALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered solution containing 100 mM Glycine,150 mM NaCl, 5% mannitol and 0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
TGF-β3 orchestrates critical processes in wound healing, chondrogenesis, and palatogenesis through high-affinity engagement of type I and type II serine/threonine kinase receptors, making precise quantification of its bioactivity essential for mechanistic studies. This tag-free recombinant protein, spanning the mature domain (residues 301–412), demonstrates potent growth-inhibitory activity with an ED50 ≤0.2 ng/mL in TF-1 cell proliferation assays conducted in the presence of IL-4, providing a validated tool for receptor-ligand binding competition assays, ELISA standard curve generation, and antibody epitope mapping experiments. Mammalian cell expression preserves the native disulfide architecture and any post-translational modifications required for full receptor engagement, supporting its use in chondrogenic and osteogenic differentiation protocols where even subtle conformational differences can alter lineage commitment. Purity exceeding 95% by SDS-PAGE and endotoxin levels ≤10 EU/mg satisfy the quality thresholds commonly required for stem cell differentiation studies and in vivo wound healing models where contamination could confound TGF-β signaling readouts.
