Abbreviation
Recombinant Human PRLR protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PRLR at 2 μg/mL can bind Anti-PRLR recombinant antibody (CSB-RA018727A0HU), the EC50 is 126.8-171.9 ng/mL.
Alternative Names
PRLR; Prolactin receptor; PRL-R
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
25-234aa
Target Protein Sequence
QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMND
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PRLR at 2 μg/ml can bind Anti-PRLR recombinant antibody (CSB-RA018727A0HU), the EC50 is 126.8-171.9 ng/mL.
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The purity of PRLR was greater than 95% as determined by SEC-HPLC
Description
The extracellular domain of human prolactin receptor (residues 25–234) encompasses the complete ligand-binding region critical for PRL–PRLR interaction studies, making this construct particularly well-suited for probing receptor-ligand dynamics without the complexity of full-length membrane protein handling. Functional ELISA validation confirms robust binding activity, with an EC50 of 126.8–171.9 ng/mL against an anti-PRLR recombinant antibody, providing direct evidence that this protein supports use in competitive inhibition assays, blocking antibody screening, and therapeutic antibody epitope mapping. Expression in mammalian cells preserves native glycosylation and disulfide bonding essential for proper folding of the fibronectin type III–like domains, which aligns with standards expected in SPR- and BLI-based affinity characterization where conformational integrity directly impacts kinetic measurements. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for drug discovery screening campaigns targeting PRL signaling and for use as a reliable positive control in binding assays.
