Abbreviation
Recombinant Human NRG1 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
≤10 EU/mg by the LAL method
Activity
Measured in a cell proliferation assay using MCF-7 breast cancer cell line. The ED50 for this effect is 1-8 ng/mL.
Alternative Names
Pro-neuregulin-1; Neuregulin-1 beta 1; NRG1-beta 1; HRG1-beta 1; EGF;NRG1; GGF; HGL; HRGA; NDF; SMDF
Species
Homo sapiens (Human)
Expression Region
20-242aa
Target Protein Sequence
SGKKPESAAGSQSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCQPGFTGARCTENVPMKVQNQEKAEELYQKR
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
Neuregulin-1 beta 1 drives ErbB receptor-mediated signaling cascades central to cardiac development, neural differentiation, and breast epithelial cell proliferation, making it a critical tool for dissecting these pathways in vitro. This mammalian-expressed fragment spanning residues 20–242 retains native post-translational modifications that support robust receptor engagement, demonstrated by an ED50 of 1–8 ng/mL in MCF-7 breast cancer cell proliferation assays—a potency range that enables dose-response studies in ErbB3/ErbB4-dependent models. The quantified activity provides a suitable basis for cell survival and differentiation experiments, including neuronal and stem cell lineage commitment assays, as well as receptor-ligand binding studies by ELISA or surface plasmon resonance. Endotoxin levels ≤10 EU/mg and purity exceeding 95% align with standards expected in sensitive primary cell cultures and functional assays where contaminant interference must be minimized.
