Abbreviation
Recombinant Human NRG1 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of >2.5x104 IU/mg.
Research Area
Neuroscience?
Alternative Names
Acetylcholine receptor-inducing activity; Acetylcholine receptor-inducing activity, chick, homolog of; ARIA; Breast cancer cell differentiation factor p45; GGF; GGF2; glial growth factor 2; Glial growth factor; Heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); heregulin, alpha; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; Neu differentiation factor; Neuregulin-1; nrg1; NRG1-IT2; NRG1_HUMAN; Pro-NRG1; Sensory and motor neuron-derived factor; SMDF
Species
Homo sapiens (Human)
Expression Region
177-241aa
Target Protein Sequence
SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 m filtered 20 mM PB, pH 6.0, 150 mM NaCl
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Neuregulin-1 signals through ErbB3/ErbB4 receptor tyrosine kinases to regulate neuronal survival, myelination, and synaptic plasticity, making it a critical tool in neuroscience research. This tag-free recombinant human NRG1 fragment (177–241 aa) encompasses the EGF-like domain responsible for receptor binding and demonstrates strong potency, with an ED50 below 40 ng/mL in serum-free MCF-7 proliferation assays, corresponding to a specific activity exceeding 2.5 × 10⁴ IU/mg. The E. coli expression system yields a compact protein free of mammalian glycosylation, providing a defined and consistent ligand well suited for receptor-ligand binding studies via SPR or ELISA, neuronal differentiation protocols, and cell proliferation or survival assays where glycan heterogeneity could confound results. Purity above 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for sensitive cell-based experiments and supports use in primary neuronal culture systems.
