Abbreviation
Recombinant Human NRG1 protein, partial (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 U/mg.
Research Area
Neuroscience?
Alternative Names
Acetylcholine receptor-inducing activity; Acetylcholine receptor-inducing activity, chick, homolog of; ARIA; Breast cancer cell differentiation factor p45; GGF; GGF2; glial growth factor 2; Glial growth factor; Heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); heregulin, alpha; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; Neu differentiation factor; Neuregulin-1; nrg1; NRG1-IT2; NRG1_HUMAN; Pro-NRG1; Sensory and motor neuron-derived factor; SMDF
Species
Homo sapiens (Human)
Expression Region
177-237aa
Target Protein Sequence
SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform 12
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Neuregulin-1 signals through ErbB3/ErbB4 receptor tyrosine kinases to regulate neuronal survival, myelination, and synaptic plasticity, making it a critical tool for neuroscience and cancer biology research. This tag-free recombinant human NRG1 fragment (177–237 aa of Isoform 12) encompasses the EGF-like domain responsible for receptor binding, and its potent bioactivity is confirmed by an ED50 of less than 50 ng/mL in serum-free MCF-7 cell proliferation assays, corresponding to a specific activity exceeding 2.0 × 10⁴ IU/mg. The E. coli expression system yields a non-glycosylated protein that provides a suitable basis for receptor-ligand binding studies via SPR or ELISA, neuronal and stem cell differentiation experiments, and cell survival assays where defined, glycosylation-independent signaling is preferred. Purity above 96% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for sensitive cell-based and in vivo tumor biology models.
