Abbreviation
Recombinant Human FLT3LG protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Alternative Names
FL; Flt 3 ligand; Flt 3L; Flt3 L; FLT3 LG; Flt3 ligand; Flt3L; FLT3L_HUMAN; Flt3lg; Fms related tyrosine kinase 3 ligand; Fms-related tyrosine kinase 3 ligand; SL cytokine
Species
Homo sapiens (Human)
Expression Region
27-181aa
Target Protein Sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.0 ,lyophilized
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
FLT3 ligand drives the expansion and differentiation of hematopoietic progenitors and dendritic cell precursors, making it a critical tool for immunology research requiring precise control over proliferative signaling. This tag-free recombinant human FLT3LG (aa 27–181) demonstrates an ED50 below 1.0 ng/mL in AML5 cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg—potency that supports use in dendritic cell differentiation protocols, JAK/STAT and MAPK signaling pathway studies, and as a reference standard in cytokine detection assays. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell functional assays, while purity greater than 97% by SDS-PAGE provides a suitable basis for antibody development workflows including immunization, ELISA coating, and positive control applications. The mature extracellular domain sequence expressed in *E. coli* aligns with standards expected in in vivo models of hematopoiesis and tumor immunology.
