Abbreviation
Recombinant Human CRTAM protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CRTAM at 2 μg/mL can bind Human CADM1(CSB-MP004425HUd9) , the EC50 is 2.277-2.649 ng/mL.
Alternative Names
Class-I MHC-restricted T-cell-associated molecule;CD355
Species
Homo sapiens (Human)
Expression Region
18-287aa
Target Protein Sequence
SLTNHTETITVEEGQTLTLKCVTSLRKNSSLQWLTPSGFTIFLNEYPALKNSKYQLLHHSANQLSITVPNVTLQDEGVYKCLHYSDSVSTKEVKVIVLATPFKPILEASVIRKQNGEEHVVLMCSTMRSKPPPQITWLLGNSMEVSGGTLHEFETDGKKCNTTSTLIIHTYGKNSTVDCIIRHRGLQGRKLVAPFRFEDLVTDEETASDALERNSLSSQDPQQPTSTVSVTEDSSTSEIDKEEKEQTTQDPDLTTEANPQYLGLARKKSG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CRTAM at 2μg/mL can bind Human CADM1(CSB-MP004425HUd9)，the EC50 is 2.277-2.649 ng/mL.
Description
CRTAM mediates adhesion and cytotoxic effector functions in NK and CD8+ T cells through its interaction with CADM1, making quantitative characterization of this receptor-ligand pair essential for understanding immune synapse formation and T-cell activation. This mammalian-expressed construct spanning residues 18–287 demonstrates robust binding to human CADM1 with an EC50 of 2.277–2.649 ng/mL in functional ELISA, providing a validated reagent for ligand-binding assays, competitive inhibition studies, and therapeutic antibody epitope mapping targeting the CRTAM–CADM1 axis. The low picomolar EC50 supports use in surface plasmon resonance and biolayer interferometry experiments requiring sensitive affinity characterization, as well as in screening campaigns for biologics or small molecules that modulate this costimulatory pathway. Purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg align with standards expected in functional binding assays and antibody validation workflows where contaminant interference must be minimized.
