Abbreviation
Recombinant Human CLDN9 protein, partial-Detergent (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU). The EC50 is 2.812-3.524 ng/mL.
Alternative Names
Claudin-9; CLDN9
Species
Homo sapiens (Human)
Expression Region
2-217aa
Target Protein Sequence
ASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
50 mM HEPES，0.15 M NaCl， 0.05% DDM，0.01% CHS，pH7.5
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU). The EC50 is 2.812-3.524 ng/mL.
Description
Claudin-9 plays a critical role in tight junction assembly and barrier function, and its aberrant expression has been implicated in tumor progression and metastasis across multiple cancer types. This mammalian-expressed protein spanning residues 2–217 retains native post-translational modifications that support proper folding of the extracellular loops essential for homotypic and heterotypic claudin interactions. Functional validation demonstrates specific binding to an anti-CLDN6/9 recombinant antibody with an EC50 of 2.812–3.524 ng/mL in ELISA format, confirming that the extracellular epitopes remain accessible and correctly presented—a prerequisite for antibody development and validation studies targeting claudin family members. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein meets the quality thresholds commonly required for cell-based assays investigating tight junction dynamics, tumor cell migration and invasion, and the molecular mechanisms underlying claudin-mediated changes in epithelial barrier integrity.
