CD63 Monoclonal Antibody

Code: CSB-MA004950A1m

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Review （1）
Citation （1）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Rabbit

Raised in: Mouse

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P08962

Target Names

CD63

Alternative Names

CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Lysosome integral membrane protein 1; Limp1; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD antigen CD63

Raised in

Mouse

Species Reactivity

Human, Rabbit

Immunogen

Recombinant Human CD63 antigen protein (103-203AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal Antibody

Isotype

IgG1

Clone No.

10F11E6

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB：1	1000-1：8000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate
All lanes CD63 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 30-120 KD KDa
Observed band size: 30-120 KD KDa
Exposure time:1min
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes CD63 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 30-120 KD KDa
Observed band size: 30-120 KD KDa
Exposure time:1min
Western Blot
Positive WB detected in: A375 whole cell lysate, Rabbit spleen tissue
All lanes CD63 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 30-120 KD KDa
Observed band size: 30-120 KD KDa
Exposure time:1min`
IHC image of CSB-MA004950A1m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA004950A1m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA004950A1m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cells with CSB-MA004950A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with CSB-MA004950A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of MCF-7 cells with CSB-MA004950A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Overlay histogram showing A549 cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing Hela cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing HepG2 cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing K562 cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
1. Exosomes extracted from Hela cells
2. Exosomes extracted from Hela cells
3. Exosomes extracted from urine

Description

The CD63 monoclonal antibody is a highly specific tool used to detect the CD63 protein in various human and rabbit samples. It was generated by immunizing mice with recombinant human CD63 protein (103-203aa), followed by the fusion of immunized mouse spleen cells with myeloma cells to produce hybridomas. The hybridomas are selected to culture in the mouse abdominal cavity, leading to the production of the ascites containing CD63 monoclonal antibodies. The resulting CD63 monoclonal antibody was then screened for specificity using a variety of assays, including ELISA, WB, IHC, IF, and FC. Its purity is over 95% using protein G purified.

CD63 protein plays an important role in vesicular trafficking, antigen presentation, and cell signaling. In cells, CD63 protein interacts with other tetraspanins, integrins, and adhesion molecules to regulate the formation and stability of multimeric protein complexes. It is also involved in the internalization and sorting of plasma membrane proteins to lysosomes for degradation, and the fusion of lysosomes with autophagosomes during autophagy.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: cells

Review: Representative Western blot image of the total cell lysates and exosomes secreted by primary astrocytes in the medium showing DICAM and CD63 expressions

By Anonymous

Citations

DICAM in the Extracellular Vesicles from Astrocytes Attenuates Microglia Activation and Neuroinflammation J Han,/,2022

Target Background

Function(From Uniprot)

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Gene References into Functions

Signaling focal contacts via CD63 were identified to facilitate cell adhesion, migration and mediate extracellular matrix-physical cues to modulate hematopoietic stem cells and progenitor cells function. PMID:28566689
Human CD63-GFP expression was controlled under the rat Sox2 promoter (Sox2/human CD63-GFP), and it was expressed in undifferentiated fetal brains. PMID:29208635
our results identify the CD63-syntenin-1-ALIX complex as a key regulatory component in post-endocytic HPV trafficking. PMID:27578500
CD63 and exosome expression is altered in scleroderma dermal fibroblasts PMID:27443953
CD63 is a critical player in LMP1 exosomal trafficking and LMP1-mediated enhancement of exosome production and may play further roles in limiting downstream LMP1 signaling. PMID:27974566
TIMP1 signaling via CD63 leads to activation of hepatic stellate cells, which create an environment in the liver that increases its susceptibility to pancreatic tumor cells. PMID:27506299
Concentrations of Cd63 were elevated in gingival crevicular fluid of patients with pre-eclampsia. PMID:26988336
CD163(+) TAMs in oral premalignant lesions coexpress CD163 and STAT1, suggesting that the TAMs in oral premalignant lesions possess an M1 phenotype in a Th1-dominated micromilieu. PMID:26242181
Our findings reveal that elevated levels of TIMP-1 impact on neutrophil homeostasis via signaling through CD63. PMID:26001794
These findings indicate that rhTIMP-1 promotes clonogenic expansion and survival in human progenitors via the activation of the CD63/PI3K/pAkt signaling pathway PMID:26213230
It was shown that treatment of macrophages with anti-CD63 inhibits CCR5-mediated virus infection in a cell type-specific manner, but that no inhibition of CXCR4-tropic viruses occurs. PMID:25658293
TM4SF5 interacted with CD151, and caused the internalization of CD63 from the cell surface. PMID:25033048
Diesel exhaust exposure during exercise induces platelet activation as illustrated by a dose-response increase in the release of CD62P and CD63. PMID:25297946
Data show that CD63 is a crucial player in the regulation of the tumor cell-intrinsic metastatic potential by affecting cell plasticity. PMID:25354204
CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells. PMID:24940653
these results indicated that high glycosylation of CD63 by RPN2 is implicated in clinical outcomes in breast cancer patients PMID:24884960
Collectively, these findings indicate that CD63 may support Env-mediated fusion as well as a late (post-integration) step in the HIV-1 replication cycle. PMID:24507450
Data suggest that TIMP1 (tissue inhibitor of metalloproteinase-1) acts as chemoattractant for neural stem cells (NSC); TIMP1 enhances NSC adhesion, migration, and cytoskeletal reorganization; these effects are dependent on CD63/CD29 (integrin beta1). PMID:24635319
Timp1 is assembled in a supramolecular complex containing CD63 and beta1-integrins along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway. PMID:23522389
Antigen-induced p38 MAPK phosphorylation in human basophils essentially contributes to CD63 upregulation PMID:18727065
Loss of CD63 has a similar phenotype to loss of P-selectin itself, thus CD63 is an essential cofactor to P-selectin. PMID:21803846
shRNA knockdown in B lymphoblastoid cell line results to increased CD4(+) T-cell recognition PMID:21660937
decreased levels of CD63 were associated with distant and lymph node metastasis status and does play a direct role in human gastric carcinogenesis. PMID:21521534
C-terminal modifications that retain LMP1 in Golgi compartments preclude assembly within CD63-enriched domains and/or exosomal discharge leading to NF-kappaB overstimulation. PMID:21527913
These findings suggest that CD63 plays an early post-entry role prior to or at the reverse transcription step. PMID:21315401
ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin beta1 PMID:21149578
Surface expression of the novel CD63 variant is a distinguishing feature of mast cells, which are are stable, multiple-use cells capable of surviving and delivering several consecutive hits PMID:20337613
this work provides the first evidence of a TIMP-4/CD63 association in astrocytoma tumor cells PMID:20693981
CD63 expression results from only the anaphylactic degranulation form of histamine release. PMID:20633031
Serum sCD163 is a homogenous protein covering more than 94% of the CD163 ectodomain including the haptoglobin-hemoglobin -binding region. PMID:19581020
Results show that AP-3 is absolutely required for the delivery of CD63 to lysosomes via the trans-Golgi network. PMID:11907283
downregulation of CD63 antigen is associated with breast tumor progression PMID:12579280
possible role in HIV-1 infections specific for macrophages PMID:12610138
Post-translational modification of CD63 may be involved in the functional and morphological changes of MHC class II compartments that occur during dendritic cell maturation PMID:12755696
relationships between the expression levels of CD61, CD63, and PAC-1 on the platelet surface and the incidences of acute rejection and tubular necrosis as well as the recovery of graft function after renal transplantation PMID:12826159
Upon platelet interaction with fibrinogen, cholesterol accumulated at the tips of filopodia and at the leading edge of spreading cells; cholesterol-rich raft aggregation was accompanied by concentration of c-Src and CD63 in these cell domains PMID:12871315
The study on CD63 included its chemistry eg. if it had and O-linked carbohydrate that was digested with O-glycanase. PMID:12974720
CD63 serves as an adaptor protein that links its interaction partners to the endocytic machinery of the cell. PMID:14660791
results suggest that CD9, CD63, CD81, and CD82 could play a role in modulating the interactions between immature DCs and their environment, slowing their migratory ability. However, only CD63 would intervene in the internalization of complex antigens PMID:15130945
Constitutive expression of CD63 may indicate that this factor does not play a direct role in thyroid carcinogenesis PMID:15375577
CD63 represents an activation-induced reinforcing element, whose triggering promotes sustained and efficient T cell activation and expansion. PMID:15528334
The linkage of CD63 with PI 4-kinase may result in the recruitment of this signaling enzyme to specific membrane locations in the platelet where it influences phosphoinositide-dependent signaling and platelet spreading. PMID:15711748
This study identifies a trafficking pathway from CD63-positive multivesicular bodies to the bacterial inclusion, a novel interaction that provides essential lipids necessary for maintenance of a productive intracellular infection. PMID:16410552
CD63 is recruited to already-budded Weibel-Palade bodies by an AP-3-dependent route PMID:16683915
CD63-syntenin-1 complex is abundant on the plasma membrane PMID:16908530
CD63 is a cell surface binding partner for TIMP-1, regulating cell survival and polarization via TIMP-1 modulation of tetraspanin/integrin signaling complex. PMID:16917503
Chronic urticaria serum-induced CD63 expression assay performed on atopic whole blood by means of tricolor flow cytometry could be the most useful tool for identification of a subset of patients with autoimmune chronic urticaria. PMID:16918509
In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID:17275019
positive correlation between CD63 and erythrocyte sedimentation rate in rheumatoid arthritis PMID:17279322
Results suggest that CD63 can be a biomarker for predicting the prognosis in early stages of lung adenocarcinoma. PMID:17350713

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, extracellular exosome. Cell surface.

Protein Families

Tetraspanin (TM4SF) family

Tissue Specificity

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Database Links

HGNC: 1692

UNIGENE: Hs.445570

KEGG: hsa:967

STRING: 9606.ENSP00000257857

OMIM: 155740

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