Abbreviation
Recombinant Mouse Il6 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of >5x107 IU/mg.
Alternative Names
Il6; Il-6Interleukin-6; IL-6; B-cell hybridoma growth factor; Interleukin HP-1
Species
Mus musculus (Mouse)
Expression Region
25-211aa
Target Protein Sequence
M+FPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered solution in 30 mM Acetic Acid, pH 3.0, 150 mM NaCl, 5 % Trehalose, 0.02 % Tween-20.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interleukin-6 is a pleiotropic cytokine central to JAK/STAT3 signaling, acute-phase responses, and B-cell differentiation, making precise functional reagents essential for mechanistic immunology studies. This tag-free recombinant mouse IL-6, encompassing the full mature sequence (25–211 aa), demonstrates exceptional potency with an ED50 below 0.02 ng/mL in 7TD1 proliferation assays, corresponding to a specific activity exceeding 5 × 10⁷ IU/mg — a level that supports use in dose-dependent proliferation assays, STAT3 phosphorylation studies, and NF-κB pathway dissection at minimal working concentrations. Endotoxin levels below 1.0 EU/μg effectively mitigate LPS-driven artifacts in sensitive immune cell cultures, making this protein appropriate for primary B-cell differentiation experiments, in vivo inflammation models, and as a quantitative reference standard in cytokine detection assays. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin profile, satisfies the criteria typical for antibody validation workflows including ELISA coating and functional blocking studies.
