Mouse Interleukin 6,IL-6 ELISA Kit

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Name: Mouse Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E04639m-IS

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Code: CSB-E04639m-IS

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Citation （4）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell culture supernates

Detect Range: 3.9 pg/ml-250 pg/ml

Sensitivity: 0.975 pg/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Il6 ELISA Kit; Il-6Interleukin-6 ELISA Kit; IL-6 ELISA Kit; B-cell hybridoma growth factor ELISA Kit; Interleukin HP-1 ELISA Kit

Abbreviation

IL6

Uniprot NO.

P08505

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

3.9 pg/ml-250 pg/ml

Sensitivity

0.975 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	90
	Range %	86-96
1:4	Average %	95
	Range %	88-101
1:8	Average %	92
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

250

2.585

2.634

2.610

2.439

125

1.736

1.667

1.701

1.531

62.5

0.956

1.018

0.987

0.816

31.25

0.592

0.621

0.606

0.436

15.6

0.408

0.411

0.409

0.239

7.8

0.284

0.273

0.279

0.108

3.9

0.224

0.214

0.219

0.048

0.166

0.175

0.170

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This kit provides quantitative measurement of interleukin-6 (IL-6) in mouse samples, supporting immunology research focused on this pleiotropic cytokine's roles in inflammation, acute-phase responses, and immune regulation. Because IL-6 levels can shift dramatically — from low picogram concentrations at baseline to elevated levels during infection or chronic inflammation — the kit is designed to capture this dynamic range across diverse experimental models.

Detection Range: The 3.9–250 pg/ml range spans basal circulating IL-6 levels in healthy mice (often <20 pg/ml) through the elevated concentrations seen in LPS challenge, collagen-induced arthritis, or tumor-bearing models, minimizing the need for serial dilutions in most acute inflammation studies while still resolving subtle baseline differences.

Sensitivity: At 0.975 pg/ml, the kit detects IL-6 in unstimulated cell culture supernates and serum from naïve mice, where concentrations frequently fall below 5 pg/ml — critical for establishing true baseline values or measuring early innate immune activation.

Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernates enables researchers to correlate systemic IL-6 levels with local tissue production and in vitro macrophage or T cell stimulation data within a single assay platform.

Assay Efficiency: Requiring only 50–100 µl per well and 1–5 hours to complete, the kit accommodates limited-volume samples from retro-orbital bleeds or primary splenocyte cultures while supporting same-day data acquisition.

This kit is particularly suited for studies in sepsis modeling, colitis-associated tumorigenesis, and Th17 differentiation pathways.

■ Customer Reviews

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The Synergistic Effects of Next-Generation Probiotics Supplementation and Anti-PD-1 Therapy Against Colorectal Cancer Through 16S rRNA Sequencing of Gut microbiota and 4 serum untargeted metabolomics Y Xiang,/,/
Surface Display of Type 1 Fimbriae on Shigella flexneri Induces Antigen-Specific Immune Response via Oral Route S Sang, R Yu, Y Mao, Y Zhai, C Cao, K Li, Y Guan,/,2025
Two-plex in vivo molecular imaging in the second near-infrared window for immunotherapeutic response Y Sun, R Li, Y Cai, Y Liu, P Wang, M Wu,/,2025
Wenqing Yin Attenuates the Allergic Contact Dermatitis Response by Inhibiting the JAK2/STAT1 and MAPK Signaling Pathways M Ren, S Li, S Li, Z Tan, J Shi,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable).; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.

Gene References into Functions

These results suggest that TCF21 contributes to the proinflammatory environment in VIS fat depots and to active ECM remodeling of these depots by regulating IL6 expression and MMP-dependent ECM remodeling in a spatiotemporally coordinated manner. PMID:29540474
Notch signaling regulates cell density-dependent apoptosis through IL-6/STAT3-dependent mechanism. PMID:30249464
Obesity-induced IL-6 shifts macrophage polarization towards tumor-promoting macrophages that produce the CCL-20 in the colitis-associated colorectal cancer (CAC) microenvironment. CCL-20 promotes CAC progression by recruiting CCR6-expressing B-cells and gammadelta T cells via chemotaxis. PMID:29695802
In glioblastoma, colony-stimulating factor-1 and angiocrine IL-6 induce robust arginase-1 expression and macrophage alternative activation, mediated through peroxisome proliferator-activated receptor-gamma-dependent transcriptional activation of hypoxia-inducible factor-2alpha. PMID:29422647
microbiota provide a homeostatic role for epithelial barrier function through regulation of intraepithelial lymphocytes -derived IL-6 PMID:28812548
Ischemia augments alloimmune injury through IL-6-driven CD4+ alloreactivity. PMID:29410442
Results suggest that IL-6 contributes to limit lipid metabolic disorder, cardiac hypertrophy, fibrosis, inflammation and myocardium lipotoxicity during HFD-induced obesity. PMID:28844956
CD37 may protect against IgA nephropathy by inhibition of the IL-6 pathway. PMID:29551516
IL-6/Stat3 signaling drives a transcriptional program of antimicrobial gene expression in infected urothelium, with key roles in limiting epithelial invasion and ascending infection. PMID:29475562
IL-6 and STAT3 have roles in potentiating FGF19-driven hepatocellular carcinoma (HCC) in mice; this finding may have translational relevance in HCC pathogenesis PMID:28508871
IGF-1R signalling contributes to T cell dependent inflammation in arthritis. Inhibition of IGF-1R on the level of insulin receptor substrates alleviates arthritis by restricting IL6-dependent formation of Th17 cells and may open for new treatment strategies in rheumatoid arthritis. PMID:28583713
The present study demonstrates for the first time that IL-6 trans-signaling is involved in the pathomechanisms of compromised fracture healing after severe injury, whereas IL-6 classic signaling rather mediates pro-regenerative effects augmenting bone regeneration PMID:29497762
persistent stimulation with titanium particles may lead to a consistent release of TNF-alpha and IL-6 via SPHK-2 activity, which may lead to aseptic implant loosening PMID:29728804
IL-6-STAT3 signaling facilitates TRIM28 binding to the Il17-Il17f locus, and this process is required for epigenetic activation and high-order chromosomal interaction in autoimmune experimental encephalomyelitis. PMID:29651155
IL-6 induces utrophin expression through NRG-1/ErbB pathway in dystrophic myotubes. PMID:27988307
IL-6 overexpression could enhance cardiomyocyte proliferation and relevant protein expression in mice myocardium, thus promoting cardiac regeneration. PMID:29966974
data revealed that IL-6 regulates the excessive release of NO through IL-1beta inhibition and determines the establishment of an M2 macrophage profile within infected heart tissue. PMID:28087471
IL-6 plays a role in consolidation process. PMID:29619678
IL-6 expands dendritic cell and monocytic populations and ultimately leads to a robust T-cell driven immune response in Complete Freund's Adjuvant immunized mice. PMID:28474508
These results suggested that the thrombinstimulated synthesis of IL6 was limited by HSP90 in osteoblasts, and that the effects of HSP90 were exerted at the point between Rhokinase and p38 MAPK. PMID:30066835
Results suggest that interleukin 6 (IL-6) may be exploited for lung repair during influenza infection. PMID:28262742
YY1 was progressively up-regulated in BV2 microglial cells stimulated with lipopolysaccharide (LPS), which was dependent on the transactivation function of nuclear factor kappa B (NF-kappaB). Furthermore, YY1 knockdown notably inhibited LPS-induced the activation of NF-kappaB signaling and interleukin-6 (IL-6) expression in BV-2cells. PMID:29803672
The authors conclude that Mycobacterium tuberculosis ESAT-6 stimulates macrophage IL-6 production through STAT3 activation. PMID:28106119
IL-6/soluble IL-6R differentially regulate RANKL-induced osteoclast differentiation and activity through modulation of NF-kappaB, ERK and JNK signaling pathways. PMID:28128332
These results suggest that the inhibition of IL6/STAT3 signaling is a potential mechanism by which PZH is used in the treatment of ulcerative colitis. PMID:29845215
CXCL9 may promote prostate cancer progression via inhibition of cytokines from T cells. PMID:29901197
results suggest that IL-6 gene requires up-regulation of phospho-JAK2/STAT3, PACAP, and PAC1R and down-regulation of the TNF-alpha gene to modulate its anticonvulsive/neuroprotective potential PMID:29673861
Toll-like receptor 4 (TLR4) requires physical and functional association with Fcalpha-mu protein (Fcalpha/muR) for its oligomer formation and interleukin-6 (IL-6) production from marginal zone (MZ) B cells. PMID:27146354
TRYP improves the health condition of mice with DSS induced colitis by regulating the TNF-α/NF-κBp65 and IL-6/STAT3 signaling pathways via inhibiting the degradation of IκBα and the phosphorylation of STAT3. PMID:29724065
Our results demonstrate that IL-6 activation in placenta is required for relaying inflammatory signals to the fetal brain and impacting behaviors and neuropathologies relevant to neurodevelopmental disease. PMID:27838335
An IL-6 infusion model can initiate macrophage accumulation as well as aortic dilation, and under conditions of elevated tension, this proinflammatory cytokine can be produced by aortic vascular smooth muscle cells. PMID:29107003
miR-155 seems to target Est-1 and induces ulcerative colitis via the IL-23/17/6-mediated Th17 pathway. PMID:28888763
IL-6 and aging are involved in regulation of PPARalpha and PGC-1alpha expression and may influence the mitochondrial function. PMID:29173012
CGRPinduced IL6 mRNA expression was mediated by mmu_circRNA_007893. PMID:29039515
The results of this study indicated that persistently increased levels of IL-6 can lead to alterations in mTOR regulation of L-LTP. PMID:29104031
These data demonstrate that the Pb18 strain of Paracoccidioides brasiliensis is able to activate the transcription of Notch1 receptor in J774 macrophages. Activation of this receptor with also activation of TLR 4 (via LPS) induces IL-6 production, which favors the pathogenesis. PMID:28600728
IRF-1 may be at the nexus of the interplay between IFN-gamma and IL-6 in exacerbating a xenobiotic-induced inflammatory response, regulation of interferon responsive genes and autoimmunity PMID:28453771
IL 6 and TGF beta perform essential role in cerebral malaria pathogenesis by modulating the level of glial cell induced neuroinflammation. PMID:28803696
These results suggest that glucocorticoids' effects on adipose tissue immune response, both in a pro- and an anti-inflammatory manner, depend on the nutritional status. PMID:29847081
EMMPRIN inhibited bFGF-induced IL-6 secretion by reducing the p65 subunit phosphorylation, it might be concluded that bFGF and EMMPRIN crosstalk in their respective signaling pathways. PMID:29104472
pathologic levels of IL-6 in the periphery may play a role in the development of seizures when viral replication within the brain is limited following infection with a variant of Theiler's murine encephalomyelitis virus that does not replicate within the parenchyma of the brain. PMID:28741149
TIARP independently down-regulated CXCL2 and IL-6 production by fibroblast-like synoviocytes, and the expression of chemokine receptors (CXCR1 and CXCR2) in neutrophils, with resultant reduction of neutrophil migration into arthritic joints. PMID:27995997
these findings revealed a novel and unexpected role of IL-6 in ameliorating acute liver injury via regulating inflammatory responses in hepatic macrophages PMID:28822324
increased circulating levels of IL-6 perturb the redox signaling cascade, even prior to the necrotic stage, leading to severe features and progressive nature of muscular dystrophy. PMID:28845212
LPS increased mRNA and protein expressions of IL-6 and RANKL on day 14 PMID:28637991
role in keratinocyte migration and proliferation through modulation of TGF-betaR expression and function PMID:27892604
The in vitro findings suggest that GTS-21-induced IL-6 release from muscle is mediated via alpha7AChRs upstream of Stat-3 and -5 pathways and is associated with myonuclear accretion, possibly via MyoD and Pax7 expression. PMID:28282360
Burn serum caused muscle cell death associated with increased mitochondrial fission and functional impairment. This alteration was alleviated with IL-6 antibody treatment, suggesting the cytokine plays a role in mitochondrial changes in muscle after systemic injury. PMID:28181922
This study demonstrates that obesity-associated inflammation and metabolic disturbances depend on interleukin-6/Stat3-dependent formation of a distinct natural killer population, which may provide a target for the treatment of obesity, metaflammation-associated pathologies, and diabetes. PMID:28683285
In the CNS, LPS administration had the greatest effect on IL-6 and LPS increased IL-6 mRNA expression only in non-neuronal cells. PMID:28456715

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Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Tissue Specificity

Expressed by dendritic cells and macrophages. Expressed by activated follicular B cells. Abundantly expressed in the central nervous system (CNS), particularly the hypothalamic region.

Database Links

UNIGENE: Mm.1019

KEGG: mmu:16193

STRING: 10090.ENSMUSP00000026845

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