Abbreviation
Recombinant Human IL36B protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Alternative Names
2310043N20Rik; Family of interleukin 1 eta; FIL1 (ETA); FIL1; FIL1 eta; FIL1H; FILI-(ETA); IL 1F8 (FIL1 eta; IL 1F8; IL 1H2; IL-1 eta; IL-1F8; IL-1H2; IL1 ETA; IL1F8 (Canonical product IL 1F8a); IL1F8; IL1F8_HUMAN; IL1H2; IL36 beta; IL36B; Interleukin 1 family, member 8 (eta); Interleukin 1 family, member 8; Interleukin 1 homolog 2; Interleukin 1 Superfamily e; Interleukin 36 beta; Interleukin-1 eta; Interleukin-1 family member 8; Interleukin-1 homolog 2; MGC126880; MGC126882; OTTMUSP00000012797; RP23-176J12.1
Species
Homo sapiens (Human)
Expression Region
5-157aa
Target Protein Sequence
REAAPKSYAI RDSRQMVWVL SGNSLIAAPL SRSIKPVTLH LIACRDTEFS DKEKGNMVYL GIKGKDLCLF CAEIQGKPTL QLKEKNIMDL YVEKKAQKPF LFFHNKEGST SVFQSVSYPG WFIATSTTSG QPIFLTKERG ITNNTNFYLD SVE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform 2
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered 2 x PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-36β (IL-1F8) signals through the IL-36 receptor to activate NF-κB and MAPK pathways, playing a central role in epithelial and immune cell inflammatory responses. This tag-free recombinant human IL-36β, spanning residues 5–157 of Isoform 2, faithfully represents the processed mature sequence and demonstrates strong signaling potency with an ED50 below 10 ng/mL in an IL-8 secretion assay using human preadipocytes, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg. These activity data support use in cell-based functional assays measuring NF-κB or MAPK activation, downstream cytokine release studies, in vivo inflammation models, and as a quantitative reference standard in cytokine detection platforms such as ELISA. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments, while purity greater than 97% by SDS-PAGE provides a suitable basis for antibody development workflows including immunization and positive control applications.
