Abbreviation
Recombinant Human IL36B protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36β at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
Alternative Names
2310043N20Rik; Family of interleukin 1 eta; FIL1 (ETA); FIL1; FIL1 eta; FIL1H; FILI-(ETA); IL 1F8 (FIL1 eta; IL 1F8; IL 1H2; IL-1 eta; IL-1F8; IL-1H2; IL1 ETA; IL1F8 (Canonical product IL 1F8a); IL1F8; IL1F8_HUMAN; IL1H2; IL36 beta; IL36B; Interleukin 1 family, member 8 (eta); Interleukin 1 family, member 8; Interleukin 1 homolog 2; Interleukin 1 Superfamily e; Interleukin 36 beta; Interleukin-1 eta; Interleukin-1 family member 8; Interleukin-1 homolog 2; MGC126880; MGC126882; OTTMUSP00000012797; RP23-176J12.1
Species
Homo sapiens (Human)
Expression Region
1-157aa
Target Protein Sequence
MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Isoform 2
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-36β (IL-1F8) signals through the IL-1Rrp2/IL-1RAcP receptor complex to drive NF-κB and MAPK activation, making it a key mediator in epithelial and immune cell inflammatory responses. This tag-free recombinant human IL-36β encompasses the full-length isoform 2 sequence (1–157 aa), and its biological activity has been confirmed by functional ELISA demonstrating that immobilized protein at 1 µg/mL binds recombinant human IL-1Rrp2 Fc chimera across a 0.15–5 µg/mL range, supporting use as a coating antigen for antibody validation, a positive control in cytokine detection assays, and a ligand in receptor-binding studies. Endotoxin levels below 1.0 EU/µg minimize LPS-driven artifacts, which provides a suitable basis for cell-based NF-κB reporter assays, immune cell activation experiments, and in vivo inflammation models where spurious innate immune signaling must be excluded. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, aligns with standards expected in quantitative signaling pathway studies and reference standard applications.
