Abbreviation
Recombinant Human IL17A protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of >1.3x105 IU/mg.
Alternative Names
CTLA 8; CTLA-8; CTLA8; cytotoxic T lymphocyte associated antigen 8; Cytotoxic T lymphocyte associated protein 8; Cytotoxic T lymphocyte associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL 17; IL 17A; IL-17; IL-17A; IL17; IL17_HUMAN; Il17a; Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8); interleukin 17A; Interleukin-17A; interleukin17; Interleukin17A; OTTHUMP00000016597; OTTMUSP00000046003
Species
Homo sapiens (Human)
Expression Region
24-155aa
Target Protein Sequence
GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
15.1 kDa/30.2kDa
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-17A is a pivotal pro-inflammatory cytokine that drives NF-κB and MAPK signaling in stromal and epithelial cells, making it central to studies of autoimmunity, host defense, and tumor microenvironment biology. This tag-free recombinant human IL-17A, encompassing the full mature sequence (residues 24–155), demonstrates robust signaling potency with an ED50 below 7.5 ng/mL in an NIH/3T3 IL-6 secretion assay, corresponding to a specific activity exceeding 1.3 × 10⁵ IU/mg — providing a reliable basis for cell-based functional assays, downstream pathway activation studies, and use as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize the risk of LPS-driven artifacts in sensitive immune cell experiments, supporting use in primary cell activation assays and in vivo inflammation models. Purity above 95% by SDS-PAGE, combined with this stringent endotoxin profile, also satisfies the criteria typical for antibody development workflows including immunization, ELISA coating, and positive control applications.
