Human Interleukin 17A (IL-17A/IL-17) ELISA Kit

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Name: Human Interleukin 17A (IL-17A/IL-17) ELISA Kit
Brand: CUSABIO
SKU: CSB-E12819h-IS

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Code: CSB-E12819h-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 15.6 pg/ml-1000 pg/ml

Sensitivity: 3.9 pg/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

CTLA 8 ELISA Kit; CTLA-8 ELISA Kit; CTLA8 ELISA Kit; cytotoxic T lymphocyte associated antigen 8 ELISA Kit; Cytotoxic T lymphocyte associated protein 8 ELISA Kit; Cytotoxic T lymphocyte associated serine esterase 8 ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; IL 17 ELISA Kit; IL 17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; IL17 ELISA Kit; IL17_HUMAN ELISA Kit; Il17a ELISA Kit; Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) ELISA Kit; interleukin 17A ELISA Kit; Interleukin-17A ELISA Kit; interleukin17 ELISA Kit; Interleukin17A ELISA Kit; OTTHUMP00000016597 ELISA Kit; OTTMUSP00000046003 ELISA Kit

Abbreviation

IL17A

Uniprot NO.

Q16552

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

3.9 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL17A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-106
1:2	Average %	94
	Range %	89-100
1:4	Average %	98
	Range %	92-105
1:8	Average %	93
	Range %	87-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

3.369

3.194

3.281

3.168

500

2.596

2.667

2.631

2.518

250

1.616

1.628

1.622

1.508

125

0.952

0.939

0.946

0.832

62.5

0.541

0.521

0.531

0.417

31.2

0.299

0.313

0.306

0.193

15.6

0.224

0.214

0.219

0.105

0.112

0.115

0.114

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of interleukin-17A (IL-17A) in human samples, supporting research into Th17-driven immune responses and inflammatory pathology. Because IL-17A levels can vary dramatically between healthy baseline and active disease states — and often fall in the low picogram range in circulation — accurate quantification demands both broad dynamic range and low-end sensitivity.

Detection Range: The 15.6–1000 pg/ml range spans from near-baseline serum IL-17A concentrations in healthy individuals to the elevated levels observed in active psoriasis, rheumatoid arthritis, and IBD flares. Most clinical and in vitro samples fall within this window without requiring extensive dilution, though stimulated Th17 culture supernates may need pre-dilution.

Sensitivity: At 3.9 pg/ml, the kit detects IL-17A in low-abundance contexts such as unstimulated PBMC conditioned media, early-stage autoimmune serum samples, or plasma from patients prior to disease flare, where circulating levels often hover in the single-digit picogram range.

Sample Compatibility: Validation across serum, plasma, cell culture supernates, and tissue homogenates enables researchers to correlate systemic IL-17A levels with local tissue expression and in vitro Th17 polarization output within a single experimental framework.

Assay Efficiency: The 1–5 hour protocol using 50–100 µl sample volumes accommodates limited clinical specimens and primary Th17 differentiation cultures where cell yields restrict supernate availability.

This kit is well-suited for studies in psoriasis immunopathogenesis, anti-IL-17 therapeutic monitoring, and Th17 differentiation profiling.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis. As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release. In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms. Enhances immunity against West Nile virus by promoting T cell cytotoxicity. May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung. Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense.

Gene References into Functions

that proliferation potential-related protein promotes esophageal cancer cell proliferation and migration, and suppresses apoptosis by mediating the expression of p53 and IL-17 PMID:30223275
The pooled estimate revealed an association between IL-17A rs2275913 polymorphism and the risk of gastric cancer (GC) under all genetic models (A vs. G, OR 1.187, 95% CI 1.086-1.297, P < 0.001; GA vs. GG, OR 1.108, 95% CI 1.008-1.218, P = 0.033; AA vs. GG, OR 1.484, 95% CI 1.236-1.781, P < 0.001), while no evidence of association was found with IL-17A rs3748067 or IL-17F rs763780 polymorphisms. PMID:29860554
Over-expression of IL-17 and IL-27 are involved in the pathogenesis of liver damage in children with human cytomegalovrius infection. PMID:30022757
IL23 and IL17 have roles in the pathogenesis of Tunisian pemphigus foliaceus PMID:30116153
our findings supported the association between IL-17 SNPs and the risk of asthma in Chinese Han population from central China. GA genotype of rs3748067 and the C carries (CT+CC) of rs763780 were associated with a higher risk of asthma PMID:30036556
IL17A and HPSE may promote tumor angiogenesis and cell proliferation and invasion in cervical cancer, possibly via the NF-kappaB signaling pathway. PMID:30066843
the results suggest that IL17A (rs2275913) polymorphism is associated with the development of rheumatic heart disease in South Indian population PMID:29985710
This study demonstrated the alteration of IL-17 levels in aseptic non-vasculitic cerebral sinovenous thrombosis PMID:30246697
In this Brazilian population, TNF and IL17 gene polymorphisms responsible for the expression of important inflammatory cytokines were associated with overall spondyloarthritis and, specifically, with ankylosing spondylitis and psoriatic arthritis, regardless of gender and HLA-B27 PMID:29849482
The single nucleotide polymorphism rs2275913 in the IL-17A gene is associated with susceptibility to viral myocarditis. PMID:29530464
IL-17A197AA polymorphism is associated with the risk of colorectal cancer. PMID:29970680
our findings demonstrated that the AA genotype from the IL-17A rs2275913 SNP is positively associated with protection to active tuberculosis but related to higher disease severity in the Argentinean population. PMID:28098168
Our results from experiments suggest that the effects of IL-17 mediate activation of STAT3 signaling in breast cancer cells. Taken together, our study shows that myeloid-derived suppressor cells can be a new type of prognostic marker in breast cancer patients. Targeting IL-17/Stat3 signaling may be a promising strategy for BC treatment. PMID:29655056
This study draws two main conclusions: 1) The presence of IL-17 polymorphism rs2275913 is closely related to a more severe form of the disease and as a result, a higher number of disease-modifying anti rheumatic drugs required to control it, 2) The presence of IL-17 polymorphism rs2275913 may confer a risk of developing rheumatoid arthritis in Mexican carriers PMID:28379210
Polymorphisms of IL-17 are associated with host susceptibility to some bacterial pathogen. [review] PMID:29345518
secreted IL-17A is not responsible for the second hit in acute pancreatitis PMID:29422392
In carriage, an increased IL-17 and IFN-gamma levels were observed following stimulation with S. aureus strains. PMID:29311230
IL17A G197A is associated with a higher susceptibility of developing OLP and these patients seem to present a considerable increase in IL17A serum levels. PMID:28741807
These findings highlight a regulatory pathway of Tiam1/Rac1 in Th17 cells and suggest that it may be a therapeutic target in multiple sclerosis. PMID:27725632
Interleukin 17A (IL-17[G197G]) was associated with preterm birth (PTB), and the PTB group had lower IL-17A expression compared to the full-term group . PMID:29431293
In studies of mouse and human pancreatic tumors and precursors, we found that immune cell-derived IL17 regulated development of tuft cells and stem cell features of pancreatic cancer cells via increased expression of DCLK1, POU2F3, ALDH1A1, and IL17RC. PMID:29604293
Expression of miR-135a in the cancer cells isolated from nasopharyngeal tumors was significantly lower than that in NP69 cells, and suppression of IL-17 by miR-135a mimic resulted in significant inhibition of NPC cell proliferation. PMID:29734196
The expression of IL-17 and IL-12 in patients with lupus miliaris disseminatus faciei is reported in patients and healthy controls. PMID:27515793
these findings suggest that the variants +2199 A/C IL-23R and -197 G/A IL-17A could contribute to rheumatoid arthritis development in the studied population PMID:28547498
IL-17A rs2275913 polymorphism did not seem to influence RA susceptibility in Tunisian population. PMID:29584788
results suggest that IL-17A stimulated keratinocytes activated PI3K/AKT/mTOR signaling and inhibited autophagy by simultaneously inhibiting autophagosome formation and enhancing autophagic flux. PMID:29432814
In the present review, we have discussed the cellular sources, modes of action and regulation of IL-17 and IL-33 in the context of hypersensitive diseases [Review] PMID:29153708
Findings identify interleukin-17A as a potential mediator of neuroanatomical remodeling of the gut innervation during inflammatory bowel diseases. PMID:28560787
This review discusses recent discoveries related to the pro-inflammatory cytokine IL17A, and its potential role in the pathogenesis of COPD. We propose that an intervention strategy targeting IL-17 signaling offers an exciting opportunity to mitigate inflammatory processes, and prevent the progression of tissue pathologies associated with COPD [Review] PMID:28438639
rs2275913 gene polymorphism associated with a risk of Bacillus-Calmette-Guerin osteitis after vaccination PMID:28731539
Study shows that higher baseline levels of Interleukin 17 are selectively associated with greater symptomatic reduction in depressed patients treated with bupropion-SSRI combination. PMID:28698115
IL-23/IL-17 axis and biochemical markers in the pathogenesis of Type 2 Diabetes PMID:28757426
Higher levels of TGF-beta mRNA were observed in biopsies taken from healthy controls, and the IL-23 mRNA levels were significantly increased in the peri-implantitis group (P < 0.0001). No differences in IL-17 mRNA levels were observed between the two groups (P > 0.05). PMID:27062688
Studied interleukin-17 (IL-17) expression levels in blood and skin of atopic dermatitis (AD) patients and controls. PMID:28279075
semen IL-17 and IL-18 levels in diabetes mellitus males were significantly higher than those in normal males and were positively correlated with blood glucose level and sperm DNA fragmentation index PMID:28858634
clinical significance of IL-17 and IL-23 in the pathogenesis of different types of gastric neoplasms in humans, is reported. PMID:27869179
The IL-17A (-832A/G) polymorphism was not associated with accelerated silicosis. PMID:28481151
IL-17A was significantly upregulated in patients with the uncontrolled and refractory status. Therefore, IL-17A may play important roles in asthmatic exacerbation, and its high level, in combination with upregulated Th2 and other cytokines, may indicate the refractory endotype of asthma. PMID:28840844
results suggest that IL17A participates in the immune response of the human host against M. tuberculosis through the activation of the autophagy process in correlation with the severity of the disease PMID:28581888
developed ultrasensitive methods for measuring IL-17A and IL-17F in human serum samples and found that serum from psoriasis patients had higher and a broader range of concentrations of both IL-17 proteins compared to healthy volunteers PMID:28534291
SNPs of rs3819024 in IL-17A and rs763780 in IL-17F were weakly related to a prognosis of tuberculosis. PMID:27339100
this study shows that aberrant NKG2D expression with IL-17 production of CD4+ T subsets in patients with type 2 diabetes PMID:27168217
Luciferase assay using the 5'-UTR of the IL-17 F gene revealed transcriptional regulation. Induced IL-17 F production was further confirmed at the protein level by ELISA. Smad1/5/8 inhibitor pretreatment decreased IL-17 F expression levels in the cells. PMID:28812969
Study shows increased IL-17A expression in early tendinopathy and proposes IL-17A as an inflammatory regulator in tendon remodeling. PMID:27263531
Several studies identified IL-17A as a pro-inflammatory player in atherosclerosis while its expression was associated with increased inflammation and plaque vulnerability in human atherosclerotic lesions. Moreover, IL-17A induced a pro-inflammatory, pro-thrombotic, plaque-destabilizing, and cell-attracting response of the inflammatory milieu of human plaque tissue samples. [review] PMID:28034277
asymmetric cell divisions in psoriasis are IL17A-dependent. PMID:28600817
Serum IL-23 and IL-17 levels were elevated in patients with aneurysmal subarachnoid hemorrhage (aSAH) showing upregulation of IL-23/IL-17 inflammatory axis after aSAH. Serum IL-23 and IL-17 showed differential correlations with post hemorrhagic complications and no correlation with clinical outcome. PMID:28609751
Data showed that IL-17A levels were sustained in respiratory samples from cystic fibrosis patients infected by P. aeruginosa. IL-17 mediated-immunity plays a double-edged found during chronic airways infection: in one hand, it contributes to the control of P. aeruginosa burden, modulating host resistance, while, on the other, it alters host tolerance, propagating exacerbated pulmonary neutrophilia and tissue remodeling. PMID:27189736
gamma delta T cells, rather than Th17cells, are the principal producers of IL-17 in acute gouty arthritis patients during the acute gout flares. PMID:29476737
high expression of both IL17A and IL32 leads to enhancement of T cell responses in breast tumors PMID:28470472