Rat interferon-inducible protein 10,IP-10 ELISA Kit

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Rat interferon-inducible protein 10,IP-10 ELISA Kit

Name: Rat interferon-inducible protein 10,IP-10 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08182r

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Code: CSB-E08182r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 781.25 pg/mL-50000 pg/mL

Sensitivity: 195 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit

Abbreviation

CXCL10

Uniprot NO.

P48973

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

781.25 pg/mL-50000 pg/mL

Sensitivity

195 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	94
	Range %	90-98
1:4	Average %	99
	Range %	95-104
1:8	Average %	102
	Range %	96-108

Recovery

The recovery of rat IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	100	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50000	2.556	2.581	2.569	2.437
25000	1.748	1.769	1.759	1.627
12500	1.002	1.085	1.044	0.912
6250	0.663	0.699	0.681	0.549
3125	0.396	0.387	0.392	0.260
1562.5	0.284	0.271	0.278	0.146
781.25	0.219	0.230	0.225	0.093
0	0.131	0.133	0.132

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Chemokine (C-X-C motif) ligand 10 (CXCL10), also known as interferon-inducible protein 10 (IP-10), is a small cytokine belonging to the CXC chemokine family. This protein attracts and activates immune cells, particularly T cells, monocytes, and natural killer cells. Interferon-gamma primarily induces CXCL10, which participates in inflammatory processes, antimicrobial responses, and tissue repair mechanisms. Its expression increases during infections, autoimmune diseases, and various pathological conditions, making it a valuable biomarker for studying immune system activation and inflammatory responses in research settings.

The Rat interferon-inducible protein 10,IP-10 ELISA Kit (CSB-E08182r) is designed for quantitative measurement of CXCL10 in Rattus norvegicus samples. This sandwich ELISA works with serum, plasma, and tissue homogenates with a detection range of 781.25 pg/mL to 50000 pg/mL and sensitivity of 195 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for measuring rat CXCL10 levels in various biological samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining inflammatory responses and immune system activation across multiple tissue types and biological fluids. Studies have applied the kit to quantify chemokine levels in both central nervous system tissues and systemic circulation, supporting research into neuroinflammation and peripheral immune responses. The kit has also been used in musculoskeletal research examining inflammatory mediators in joint-related pathologies.

• Neuroinflammation research: Measurement of chemokine production in brain tissue homogenates and plasma to evaluate central nervous system inflammatory responses
• Systemic inflammation studies: Analysis of serum chemokine levels alongside other pro-inflammatory cytokines and immune mediators in comprehensive immunological studies
• Musculoskeletal research: Quantification of inflammatory mediators in both serum and articular synovial fluid to examine joint-related inflammatory processes
• Multi-biomarker profiling: Integration with panels measuring various cytokines, growth factors, and inflammatory markers to provide comprehensive immune system characterization

■ Customer Reviews

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Lipopolysaccharide worsens the prognosis of experimental cerebral ischemia via interferon gamma-induced protein 10 recruit in the acute stage Ping Wang,/,2019
Long non-coding RNA X-inactive specific transcript silencing ameliorates primary graft dysfunction following lung transplantation through microRNA-21 … Li J,/,2020
Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,/,2020
Novel In Vivo Assessment of Antimicrobial Efficacy of Ciprofloxacin Loaded Mesoporous Silica Nanoparticles against Salmonella typhimurium Infection MN Alandiyjany,/,2023
Therapeutic and prophylactic effects of baicalin on Pneumocystis infection in immunosuppressed rats T Xue, W Du, C Liu, W Dai, L Wang, P Kong,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.

Gene References into Functions

These observations provide evidence for IP-10 in a regulatory role in cold ischemia-elicited acute allograft rejection and in PRINS lncRNA expression PMID:29880386
CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID:27271043
data support our hypothesis that increased circulating IFN-gamma and glomerular TNF-alpha induce synergistically the production and secretion of CXCL10 by podocytes, attracting activated macrophages into kidney tissue. PMID:25561167
CXCL10 is a regulator for an increase of opioid-containing macrophages and antinociceptive mediator in inflammatory pain and as a key chemokine regulated by electroacupuncture PMID:24732949
CXCL10 may be a necessary algogenic molecule, especially in the development of Cancer-induced bone pain PMID:24337539
CXCL10 is involved in astrocyte migration in amyloid-beta-stimulated hippocampal astrocytes. PMID:23270423
CXCL10 is a potential candidate for the reduction in myelination in cultures on TnC-astrocytes PMID:21917786
CXCL10 and CCL5 but not CXCL11 are involved in the pathogenesis of multiple sclerosis. PMID:20720435
upregulated in endometriosis tissues PMID:20452033
interferon-inducible protein 10 (IP-10) as ethanol-responsive gene in the fetal rat brain PMID:12354640
Antibodies to this chemokine increase IFNgamma in BALF of F344 rats following Sendai virus infection. PMID:12581489
CXCL10-induced elevations in intracellular Ca2+ are associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and activity of hippocampal neurons. PMID:15465598
IP-10/CXCR3 signaling is associated with the pathogenesis of experimental autoimmune myasthenia gravis in rats. PMID:15843529
Our study demonstrates a role of IP-10/CXCL10 on T cell recruitment in a rat model of renal endothelial microvascular injury PMID:16382019
this study shows that IP-10 contributes to the regulation of the expression of SD (slit-diaphram) components not only in the physiologic state but also in pathologic states. PMID:16382022
Inhibition of CXCR3/IP-10 signaling can be considered as a potential treatment modality for myasthenia gravis. PMID:19232748