Abbreviation
Recombinant Rat Inp10 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.
Alternative Names
Cxcl10; Inp10; Mob1; Scyb10C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Interferon-inducible protein 10; Protein Mob-1; Small-inducible cytokine B10
Species
Rattus norvegicus (Rat)
Expression Region
22-98aa
Target Protein Sequence
IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL10 (IP-10) is a key interferon-γ–inducible chemokine that drives CXCR3-dependent lymphocyte trafficking, making it central to studies of inflammation, infection, and tumor immunology. This tag-free recombinant rat CXCL10, encompassing the full mature sequence (residues 22–98), demonstrates confirmed chemotactic activity at 10–50 ng/mL in a bioassay using human CXCR3-transfected HEK293 cells, providing a suitable basis for chemotaxis and migration assays, CXCR3 signaling pathway dissection (including MAPK and PI3K/Akt cascades), and in vivo rat models of inflammatory or tumor biology. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell activation readouts, which supports use in sensitive functional assays such as T-cell polarization and proliferation studies. Produced in *E. coli* with greater than 95% purity by SDS-PAGE, this protein also meets the quality thresholds commonly required for use as a reference standard in cytokine detection assays or as a positive control in ELISA and antibody validation workflows.
