Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit

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Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit

Name: Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit
Brand: CUSABIO
SKU: CSB-E16980p-IS

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Code: CSB-E16980p-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 23.5 pg/mL-1500 pg/mL

Sensitivity: 11.75 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Abbreviation

TNF

Uniprot NO.

P23563

Species

Sus scrofa (Pig)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

23.5 pg/mL-1500 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig TNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	98
	Range %	93-103
1:4	Average %	95
	Range %	90-102
1:8	Average %	93
	Range %	87-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1500

2.909

2.834

2.872

2.699

750

1.806

1.697

1.752

1.579

375

0.990

1.028

1.009

0.836

187.5

0.567

0.589

0.578

0.405

0.344

0.321

0.332

0.159

0.277

0.283

0.280

0.107

23.5

0.228

0.214

0.221

0.048

0.176

0.170

0.173

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of TNF-α in porcine samples, supporting immunology research where this master pro-inflammatory cytokine drives innate immune activation, sepsis modeling, and tissue inflammation. Because porcine TNF-α levels can shift rapidly from undetectable baseline concentrations to high nanogram ranges during acute immune challenge, the kit is designed to capture this dynamic expression across diverse experimental contexts.

Detection Range: The 23.5–1500 pg/mL range spans the concentrations typically observed in LPS-stimulated porcine whole blood, activated alveolar macrophage supernatants, and serum from experimentally induced endotoxemia, reducing the need for extensive serial dilution in most acute inflammation models.

Sensitivity: At 11.75 pg/mL, the kit detects the low constitutive TNF-α levels present in unstimulated porcine monocyte cultures and baseline serum, enabling accurate measurement of early innate immune priming before overt inflammatory amplification.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates allows researchers to correlate systemic circulating TNF-α with local production in gut, lung, or lymphoid tissue — critical for porcine mucosal immunology and organ-level inflammation studies.

Assay Efficiency: The 1–5 hour protocol using only 50–100 µL accommodates limited-volume neonatal piglet samples and high-throughput screening of multiple treatment groups common in swine vaccine trials.

This kit directly supports research in porcine sepsis modeling, PRRSV-associated immunopathology, and intestinal barrier dysfunction studies.

■ Customer Reviews

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Hydroxy-Selenomethionine Supplementation During Gestation and Lactation Improve Reproduction of Sows by Enhancing the Antioxidant Capacity and Immunity Under Heat Stress Conditions J Wang, H Sun, Z Peng, SQ Wang, YQ Yan, WC Luo,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Gene References into Functions

these findings provide the direct evidence that ADAM17 cleaves porcine TNFalpha, which represents a new view for identifying potential therapeutic targets in anti-porcine reproductive and respiratory syndrome virus therapy PMID:26724939
TMZ pretreatment effectively reduced the myocardial damage caused by CME via inhibiting the PDCD4/NF-kappaB/ TNF-alpha pathway in cardiomyocytes. PMID:28683436
TNF-alpha, secreted from activated Monocytes, mediates the downregulation of OTX2 and essential retinal pigment epithelium genes. PMID:27660103
TNF-alpha induced MMP-13 expression by condylar cells might be involved in the degradation of the juvenile condyle. PMID:27618228
TNF-alpha was able to promote theca interna cell proliferation. Our results suggest that TNF-alpha might play a role in hyperandrogenism, cortex thickness, and the increased ovary volume observed in polycystic ovaries. PMID:27125399
This study showed that the -791(C-->T) mutation of the TNF-alpha gene could be considered an important potential genetic marker of enterotoxigenic Escherichia coli F18 resistance. PMID:27408333
Both Nsp1beta and Nsp11 of porcine reproductive and respiratory syndrome virus were demonstrated to be responsible for the inhibitory effect on TNF-alpha production in pulmonary alveolar macrophages. PMID:25708177
Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID:21314908
the role of miR-181a in adipocyte differentiation by regulation of TNF-alpha PMID:24098322
The NOS inhibitor, L-NMMA, significantly suppressed the combined effects of HT and CORM-2 on TNFalpha-triggered NFkappaBp65 phosphorylation as well as decreased cell viability. PMID:24066302
High-volume hemofiltration improves hemodynamics and heart dysfunction in septic shock pigs, which may be attributed to reduction of TNF-alpha in myocardium but not in circulation. PMID:22994292
Basal lipogenesis was not affected by tumor necrosis factor alpha (TNFalpha) treatment; however, insulin stimulated lipogenesis was reduced by TNFalpha. Interleukin 6 and TNFalpha gene expression were acutely (2-4 h) stimulated by exogenous TNFalpha treatment. PMID:23090779
In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID:23102097
These data demonstrate that CRF triggers increases in intestinal paracellular permeability via mast cell dependent release of TNF-alpha and proteases. PMID:22768175
We identified critical amino acid residues in PRRSV Nsp1alpha and Nsp1beta that are important for TNF-alpha down-regulation and attenuation in vivo. PMID:22699004
Porcine reproductive and respiratory syndrome virus infection impaired TNF-alpha production by inhibiting ERK signaling. pathway. PMID:22497732
Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions. PMID:21471537
These results suggest that trans-10, cis-12-conjugated linoleic acid can modulate TNF-alpha production and NF-kappa B expression by a PPARgamma-dependent pathway in porcine peripheral blood mononuclear cells. PMID:21205430
Retinal ischemia results in increased expression of TNF-alpha and its receptors (TNF-R1 and TNF-R2). PMID:21152396
increased plasma levels during recovery from cardiac arrest are associated with left ventricle dysfunction PMID:19642909
Viral non-structural protein 1 suppressed host TNF-alpha promoter by inhibiting NF-kappaB and Sp1 promoter binding activity. PMID:20701940
Exposure of porcine oocytes to an elevated TNF-alpha concentration clearly caused a reduction in their maturation from GV stage to MII stage and increased the proportion of oocytes with abnormal chromosome alignment and cytoskeleton structure PMID:19324350
Survival in the setting of severe, acute trauma/hemorrhagic shock appears to be associated with an immediate increase in serum TNF-alpha. PMID:20027315
These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID:19605905
Repression of TNF-alpha associated with moderate hypothermia during experimental cardiac surgery is due to inhibition of the mitogen-activated protein kinase p38/activating protein-1 pathway and not to inhibition of NF-kappa B. PMID:16606437
Stenting of coronary arteries was associated with an eight-fold enhancement in TNFalpha expression in injured arterial tissue. PMID:16857205
The effect of EGF on pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and cyclooxygenase-2 (COX-2), levels during wound healing in swine is reported. PMID:17098266
In coronary microembolization, TNF-alpha is responsible for both progressive contractile dysfunction and delayed protection against infarction. PMID:17170366
The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary Escherichia coli infection. PMID:17903420
the ERK/GEF-H1/Rho/Rho kinase/phospho-MLC pathway is the mechanism mediating TNF-alpha-induced elevation of tubular epithelial permeability, which in turn might contribute to kidney injury PMID:19261619
Data show that cysteine significantly reduced the expression of pro-inflammatory cytokines, including TNF-alpha, IL-6, IL-12p40, IL-1beta, and resulted in increased expression of the apoptosis initiator caspase-8 and bcl2L1. PMID:19520150
TNF-alpha inhibits adipogenesis through stabilization of beta-catenin protein in porcine preadipocytes. PMID:19558791

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.

Protein Families

Tumor necrosis factor family

Database Links

UNIGENE: Ssc.100

KEGG: ssc:397086

STRING: 9823.ENSSSCP00000001491

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