Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

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Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

Name: Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit
Brand: CUSABIO
SKU: CSB-E17797m

CSB-E17797m

Rate （5.0）
Review （1）
Citation （1）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL

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Orders can only be placed from within the U.S.

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Alternative Names

Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit

Abbreviation

IL-18BP

Uniprot NO.

Q9Z0M9

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	84
	Range %	80-88
1:4	Average %	104
	Range %	100-107
1:8	Average %	91
	Range %	87-95

Recovery

The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	89	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.729	2.799	2.764	2.592
5	2.302	2.342	2.322	2.150
2.5	1.513	1.587	1.550	1.378
1.25	0.928	0.965	0.947	0.775
0.625	0.548	0.539	0.544	0.372
0.312	0.381	0.375	0.378	0.206
0.156	0.278	0.274	0.276	0.104
0	0.171	0.173	0.172

Troubleshooting and FAQs

ELISA kit FAQs

Datasheet & COA

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Description

This Mouse IL-18BP ELISA Kit was designed for the quantitative measurement of Mouse IL-18BP protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

■ Customer Reviews

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Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: describe

By Anonymous

IL-18 drives the Bhlhe40-mediated pathogenic Th17 cell response and exacerbates autoimmune disease progression Y Tang, Y Zhao, Z Chen, X Shi, Y Zhou, L Li,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function

Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.

Gene References into Functions

IL-18BP ameliorates cardiac ischemia/reperfusion injury in part through suppression of Th17 differentiation. PMID:26340315
Data show that IL-18bp acts as a sensor for IFN-gamma and can regulate both Th1 and Th17 responses in the CNS. PMID:20644165
Administratioan of murine IL-18BP at the time of the booster injection of bovine CII reduces the progressive severity of collagen-induced arthritis; both clinical disease activity and histological scores are decreased by 50%. PMID:12574381
functional polarization of Ag-specific CD4(+) Th2 cells entering the CNS during the accelerating phase of experimental autoimmune encephalomyelitis is flexible and dependent on IL-18, which is overturned by IL-18 binding protein PMID:15778395
IL-18 and IL-18BP may have roles in testicular development, and also in the regulation of testicular functions under physiological conditions PMID:18299271
A single intravenous dose of recombinant mouse IL18BP decreased neointimal hyperplasia and improved arterial L/A ratios in an atherosclerotic balloon-injury animal model. PMID:18455646