Mouse Thrombopoietin,TPO ELISA kit

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Name: Mouse Thrombopoietin,TPO ELISA kit
Brand: CUSABIO
SKU: CSB-E04746m-IS

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Code: CSB-E04746m-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 31.25 pg/mL-2000 pg/mL

Sensitivity: 15.6 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Thpo ELISA Kit; Thrombopoietin ELISA Kit; C-mpl ligand ELISA Kit; ML ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; MGDF ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit

Abbreviation

THPO

Uniprot NO.

P40226

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-100
1:2	Average %	97
	Range %	91-103
1:4	Average %	87
	Range %	82-95
1:8	Average %	91
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

3.261

3.114

3.187

3.064

1000

2.533

2.427

2.480

2.357

500

1.536

1.528

1.532

1.409

250

0.916

0.939

0.928

0.805

125

0.575

0.591

0.583

0.460

62.5

0.387

0.373

0.380

0.257

31.25

0.240

0.234

0.237

0.114

0.121

0.125

0.123

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

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Description

This ELISA kit provides quantitative measurement of thrombopoietin (THPO) in mouse serum, plasma, and tissue homogenates, supporting cardiovascular and hematopoietic research. Because circulating THPO levels fluctuate in response to platelet consumption, megakaryocyte mass, and vascular injury, accurate quantification across both systemic and tissue-level compartments is essential for interpreting thrombotic and cardiovascular phenotypes.

Detection Range: The 31.25–2000 pg/mL range captures both baseline murine THPO concentrations and the elevated levels observed during thrombocytopenia or myelosuppressive states. Most unstimulated mouse serum samples fall within this window without dilution, though samples from severely thrombocytopenic models may require verification at the upper boundary.

Sensitivity: At 15.6 pg/mL, the kit detects THPO in conditions where platelet counts are normal or elevated and circulating THPO is consequently low — critical for distinguishing subtle regulatory shifts in steady-state thrombopoiesis.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates allows researchers to correlate systemic THPO levels with local production in liver or bone marrow, enabling organ-specific pathway analysis in cardiovascular injury models.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate serial bleeding designs in mice, where plasma yield per draw is inherently limited.

This kit is directly applicable to studies of myocardial infarction-associated thrombocytosis, myeloproliferative neoplasm modeling, and platelet-mediated vascular repair.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Gene References into Functions

Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively... IL-11 promoted Hematopoietic stem cell engraftment in a mouse model of aplastic anemia an effect that was attenuated in cells overexpressing miR-204-5p. PMID:29217821
this study assessed the physiological source of thrombopoietin, a key cytokine required for maintaining hematopoietic stem cell . PMID:29622652
TPO and its receptor Mpl are dispensable for platelet survival in adult mice PMID:27344013
Furthermore, although the contribution of the TPO treated graft to long-term hematological engraftment was reduced, the TPO treated and untreated grafts both contributed significantly to long-term chimerism in vivo. PMID:25137252
TPO treatment also promoted the peripheral induction of Foxp3(+) Tregs in conjunction with elevated circulating TGF-beta levels. PMID:25212676
novel findings about aspects of TPO action on stem cells PMID:25564715
Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID:25339357
Thrombopoietin/MPL signaling confers growth and survival capacity to CD41-positive cells in a mouse model of Evi1 leukemia. PMID:25298035
Megakaryocytes regulate cell cycle quiescence of hematopoietic stem cell through the production of thrombopoietin. PMID:25451253
IEX-1 has a role in activation of Erk and NF-kB pathways, which affects thrombopoietin-promoted NHEJ DNA repair in hematopoietic stem cells PMID:24184684
Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID:22284746
Signal transduction pathway of ERK1/2 participates in the activation of thrombopoietin in inflammatory injury of BV2 cells. PMID:21507308
The implication of Tpo as a mediator of neuronal damage in experimental pneumococcal meningitis is reported. PMID:21149592
Administration of recombinant human IL11 after supralethal radiation exposure promotes survival in mice: interactive effect with thrombopoietin PMID:12005542
glycan domain of thrombopoietin acts in trans to enhance secretion of the hormone and other cytokines PMID:12101178
Thrombopoietin expands hematopoietic stem cells after transplantation PMID:12163458
p38 mitogen-activated protein kinase (MAPK) was responsible for the TPO-induced Hoxb4 elevation PMID:12855555
Mpl stimulation by TPO results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID:14726379
Thrombopoietin induces HOXA9 nuclear transport in immature hematopoietic cells PMID:15254242
mpl-tpo signaling pathways are negatively modulated by lnk PMID:15337790
is strongly proapoptotic in the brain, causing death of newly generated neurons through the Ras-extracellular signal-regulated kinase 1/2 pathway PMID:15642952
TPO(high) and GATA-1(low) alterations are linked in an upstream-downstream relationship along a pathobiologic pathway leading to development of myelofibrosis PMID:15665119
Development of myelofibrosis and osteosclerosis depends on local TPO levels in Bone marrow cells. PMID:15927672
Thrombopoietin synthesis is induced by IL-6 in the liver during acute inflammation. PMID:16022585
results suggest that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in HSCs PMID:17284614
thrombopoietin has a role in mast cell differentiation [review] PMID:17468237
Chronic TPO overexpression induces mesangioproliferative glomerulopathy. PMID:17546634
roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit. PMID:17925492
Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.( PMID:18410987
TPO induces HIF-1alpha expression in a manner very similar to that of hypoxia. PMID:18473128
thrombopoietin potentiates vasculogenesis by enhancing motility and enlivenment of transplanted endothelial progenitor cells via activation of Akt/mTOR/p70S6kinase signaling pathway PMID:18773906
TPO downmodulates c-Myb expression through induction of miR-150. PMID:18814950