Abbreviation
Recombinant Mouse Thpo protein, partial (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Alternative Names
Thpo; Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Species
Mus musculus (Mouse)
Expression Region
22-195aa
Target Protein Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4, with 5% Trehalose ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Thrombopoietin is the primary physiological regulator of megakaryopoiesis and platelet production, acting through the c-Mpl receptor to drive progenitor cell proliferation and differentiation. This tag-free recombinant mouse Thpo fragment (residues 22–195) encompasses the receptor-binding domain, and its potency is confirmed by an ED50 below 1.0 ng/ml in a MO7e cell proliferation assay, corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg — providing a reliable standard for megakaryocyte colony-stimulating assays, stem cell expansion studies, and receptor-ligand binding experiments using ELISA or SPR. The E. coli expression system yields a non-glycosylated protein, which is advantageous when researchers need to isolate receptor-binding kinetics from glycosylation-dependent variables, and the partial sequence focuses on the biologically active N-terminal domain responsible for c-Mpl engagement. Purity above 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for in vivo tumor biology models and sensitive cell-based proliferation assays.
