Mouse Interferon epsilon(IFNE) ELISA kit

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Code: CSB-EL011049MO

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

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Target Background

Product Details

Target Name

interferon, epsilon

Alternative Names

Ifne ELISA Kit; Ifne1 ELISA Kit; Ifnt1Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit; Interferon tau-1 ELISA Kit

Abbreviation

IFNE

Uniprot NO.

Q80ZF2

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-88
1:2	Average %	94
	Range %	89-98
1:4	Average %	103
	Range %	97-108
1:8	Average %	99
	Range %	95-104

Recovery

The recovery of mouse IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	89	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.271	2.366	2.319	2.143
500	1.563	1.625	1.594	1.418
250	0.944	0.956	0.950	0.774
125	0.582	0.581	0.582	0.406
62.5	0.382	0.377	0.380	0.204
31.2	0.274	0.289	0.282	0.106
15.6	0.220	0.235	0.228	0.052
0	0.172	0.179	0.176

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Mouse IFNE ELISA Kit was designed for the quantitative measurement of Mouse IFNE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function(From Uniprot)

Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral, including HSV-2, and bacterial, including Chlamydia muridarum, genital infections.

Gene References into Functions

IFN-epsilon is constitutively expressed by cells of the reproductive tract and is inefficiently secreted by fibroblasts and cell lines. PMID:23951133
IFN-epsilon could promote migration of antigen-specific CD8(+)T cells to the gut. PMID:22617838
Ifn-epsilon-deficient mice had increased susceptibility to infection of the female reproductive tract by the common sexually transmitted infections (STIs) herpes simplex virus 2 and Chlamydia muridarum. PMID:23449591
interactions between estradiol and IFN-tau in the context of the overall immune response during autoimmune orchitis PMID:21787220

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Subcellular Location

Secreted.

Protein Families

Alpha/beta interferon family

Tissue Specificity

Expressed at very high levels in uterus and, at much lower levels, in ovary and cervix. Very low levels, if any, in other organs. In the endometrium, expressed in the luminal and glandular epithelial cells (at protein level).

Database Links

UNIGENE: Mm.246593

KEGG: mmu:230405

STRING: 10090.ENSMUSP00000059199

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