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Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

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Code: CSB-EL009383MO

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 25 pg/mL-1600 pg/mL

Sensitivity: 6.25 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Product Details
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Target Background

Product Details

Target Name

GDNF family receptor alpha like

Alternative Names

Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit

Abbreviation

GFRAL

Uniprot NO.

Q6SJE0

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

25 pg/mL-1600 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	101
	Range %	97-107
1:4	Average %	94
	Range %	90-98
1:8	Average %	97
	Range %	92-101

Recovery

The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	88	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	1.859	1.952	1.906	1.766
800	1.258	1.260	1.259	1.119
400	0.720	0.734	0.727	0.587
200	0.466	0.489	0.478	0.338
100	0.315	0.328	0.322	0.182
50	0.229	0.235	0.232	0.092
25	0.172	0.176	0.174	0.034
0	0.138	0.141	0.140

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Mouse GFRAL ELISA Kit was designed for the quantitative measurement of Mouse GFRAL protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Citations

Hippocampal and Prefrontal Cortical Brain Tissue Levels of Irisin and GDF15 Receptor Subunits in Children TC Jackson,/,2021

Target Background

Function(From Uniprot)

Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.

Gene References into Functions

isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID:28953886

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Subcellular Location

Cell membrane; Single-pass membrane protein; Extracellular side.

Protein Families

GDNFR family

Tissue Specificity

Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.

Database Links

UNIGENE: Mm.213862

KEGG: mmu:404194

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