Human guanylate cyclase activator 2B (GUCA2B) ELISA kit

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Human guanylate cyclase activator 2B (GUCA2B) ELISA kit

Name: Human guanylate cyclase activator 2B (GUCA2B) ELISA kit
Brand: CUSABIO
SKU: CSB-EL010048HU

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Code: CSB-EL010048HU

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, urine, cell culture supernates

Detect Range: 31.25 pg/mL-2000 pg/mL

Sensitivity: 7.8 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

guanylate cyclase activator 2B

Alternative Names

GUCA2B ELISA Kit; GCAP II ELISA Kit; GCAP-II ELISA Kit; Guanylate cyclase activator 2B (uroguanylin) ELISA Kit; Guanylate cyclase activator 2B ELISA Kit; Guanylate cyclase C-activating peptide II ELISA Kit; GUC2B_HUMAN ELISA Kit; GUCA2B ELISA Kit; UGN ELISA Kit; Uroguanylin ELISA Kit

Abbreviation

GUCA2B

Uniprot NO.

Q16661

Species

Homo sapiens (Human)

Sample Type

serum, plasma, urine, cell culture supernates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
	Range %	85-91
1:10	Average %	102
	Range %	98-105
1:20	Average %	99
	Range %	93-105
1:40	Average %	95
	Range %	92-97

Recovery

The recovery of human GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-96
EDTA plasma (n=4)	92	87-96

Typical Data

Human guanylate cyclase activator 2B (GUCA2B) ELISA kit

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Human GUCA2B ELISA Kit was designed for the quantitative measurement of Human GUCA2B protein in serum, plasma, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

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Circulating Pro-Uroguanylin Levels In Children And Their Relation To Obesity, Sex And Puberty Cintia Folgueira.et al,/,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.

Gene References into Functions

The simulations suggested that all missense SNPs considered as convergent deleterious caused some kind of structural change to the uroguanylin peptide. Additionally, four of these SNPs were also shown to cause modifications in peptide flexibility, possibly resulting in functional changes. PMID:27620667
Uroguanylin concentrations are increased in patients with chronic renal failure, nephrotic syndrome, or those on dialysis. PMID:15780094
The occurrences of the C-A haplotype (rs883062-rs1047047) and the C-A-G haplotype (rs883062-rs1047047-rs2297566) were significantly higher in the essential hypertension group than in the controls. PMID:18037771

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Subcellular Location

Secreted.

Protein Families

Guanylin family

Tissue Specificity

Stomach and intestine.

Database Links

HGNC: 4683

UNIGENE: Hs.32966

KEGG: hsa:2981

STRING: 9606.ENSP00000361662