Abbreviation
Recombinant Human TSLP protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of >3.3x106 IU/mg.
Alternative Names
Thymic stromal lymphopoietin; Thymic stromal lymphopoietin protein TSLP; Tslp; TSLP protein; TSLP_HUMAN
Species
Homo sapiens (Human)
Expression Region
29-159aa
Target Protein Sequence
M+YDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150 mM NaCl
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
TSLP is a key epithelial-derived cytokine that drives Th2 polarization and dendritic cell activation, making it central to allergic inflammation and atopic disease modeling. This tag-free recombinant human TSLP, encompassing the full-length mature sequence (29–159 aa), demonstrates an ED50 below 0.3 ng/mL in a BaF3 proliferation assay co-expressing IL-7Rα and TSLPR, corresponding to a specific activity exceeding 3.3 × 10⁶ IU/mg — potency that supports use in cell-based proliferation and differentiation assays, JAK/STAT signaling pathway studies, and as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments such as dendritic cell maturation, PBMC activation, and in vivo models of allergic airway inflammation. Purity exceeding 98% by SDS-PAGE, combined with this endotoxin profile, also provides a suitable basis for antibody development workflows including immunization, ELISA coating, and positive control applications.
