Abbreviation
Recombinant Mouse Ccl9 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
Alternative Names
CCF18; Ccl9; CCL9(31-101); CCL9_MOUSE; Chemokine CC motif ligand 9; Macrophage inflammatory protein 1-gamma; Macrophage inflammatory protein-related protein 2; MIP-1-gamma; MRP-2; SCYA10; SCYA9; Small inducible cytokine A9; Small-inducible cytokine A9
Species
Mus musculus (Mouse)
Expression Region
22-122aa
Target Protein Sequence
QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL9 (MIP-1γ) plays a distinctive role in myeloid cell recruitment and tissue homeostasis, making it a key tool for dissecting chemokine-driven immune cell trafficking in murine models. This tag-free recombinant protein encompasses the full-length mature sequence (residues 22–122) and demonstrates potent chemotactic activity on human neutrophils at 0.1–10 ng/ml, providing a suitable basis for Transwell migration assays, MAPK and NF-κB signaling pathway studies, and in vivo inflammation or tumor biology models requiring precise leukocyte recruitment. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound cytokine-specific readouts in sensitive immune cell functional assays, while purity exceeding 95% by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a coating antigen for antibody development and ELISA validation. The E. coli-derived, untagged format ensures that observed biological responses reflect native CCL9 signaling without interference from fusion partners.
