Abbreviation
Recombinant Mouse Ccl6 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml.
Alternative Names
Ccl6; C10; Scya6C-C motif chemokine 6; Protein C10; Small-inducible cytokine A6) [Cleaved into: CCL6(22-95); CCL6(23-95)]
Species
Mus musculus (Mouse)
Expression Region
22-116aa
Target Protein Sequence
GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.0, 500 mM NaCl
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL6 (C10) is a murine CC chemokine that signals through CCR1 to regulate monocyte and macrophage recruitment during inflammatory and infectious responses. This tag-free recombinant protein encompasses the full-length mature sequence (residues 22–116), and its biological activity has been confirmed via chemotaxis bioassay using human CCR1-transfected BaF3 cells at an effective concentration range of 10–100 ng/mL, providing a suitable basis for chemotaxis and migration assays, downstream CCR1 signaling studies involving MAPK and NF-κB pathway activation, and in vivo mouse models of inflammation or infection. Endotoxin levels below 1.0 EU/μg minimize the risk of LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a positive control for antibody validation by ELISA and Western blot. Produced in *E. coli*, this recombinant CCL6 protein aligns with standards expected in rigorous cell-based and in vivo immunology research.
