Abbreviation
Recombinant Human CCL8 protein (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
Alternative Names
Ccl8; CCL8_HUMAN; HC14; MCP-2; MCP-2(6-76); Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; SCYA10; SCYA8; Small-inducible cytokine A8
Species
Homo sapiens (Human)
Expression Region
24-99aa
Target Protein Sequence
QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL8 (MCP-2) is a potent CC chemokine that recruits monocytes, T lymphocytes, and other immune cells through CCR1, CCR2B, and CCR5 signaling, making it a critical tool for dissecting leukocyte trafficking mechanisms. This tag-free recombinant protein encompasses the full-length mature sequence (24–99 aa) and demonstrates confirmed biological activity in chemotaxis bioassays using human peripheral blood monocytes at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, monocyte activation studies, and downstream investigation of MAPK and NF-κB signaling pathways. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound cytokine-dependent readouts in primary immune cell experiments, while purity exceeding 96% by SDS-PAGE supports use as a reference standard in chemokine detection assays, a coating antigen for antibody validation by ELISA, or a defined stimulus in in vivo inflammation models.
