Abbreviation
Recombinant Human CCL24 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-100 ng/ml.
Alternative Names
C C motif chemokine 24; C-C motif chemokine 24; CCL24; CCL24_HUMAN; Chemokine CC Motif Ligand 24; CK beta 6; CK-beta-6; Ckb6; Eosinophil chemotactic protein 2; Eotaxin-2; MPIF 2; MPIF-2; MPIF2; Myeloid progenitor inhibitory factor 2; SCYA24; Small inducible cytokine A24; Small inducible cytokine subfamily A (Cys-Cys) member 24 ; Small-inducible cytokine A24
Species
Homo sapiens (Human)
Expression Region
27-104aa
Target Protein Sequence
VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL24 (Eotaxin-2) is a potent CCR3 ligand that selectively recruits eosinophils, making it a critical tool for dissecting type 2 immune responses and allergic inflammation pathways. This tag-free recombinant protein encompasses the mature sequence (residues 27–104) and demonstrates confirmed biological activity in eosinophil chemotaxis bioassays at 50–100 ng/ml, providing a suitable basis for Transwell migration assays, CCR3 signaling studies involving MAPK and calcium flux, and in vivo models of airway or tissue eosinophilia. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound innate immune cell readouts, which supports use in sensitive functional assays with primary granulocytes as well as in antibody validation workflows such as ELISA standard curves and Western blot positive controls. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin profile, satisfies the quality thresholds commonly required for quantitative cytokine detection reference standards and dose-response characterization experiments.
