Txnip

Txnip, or thioredoxin-interacting protein, is a key regulator of cellular redox balance. It binds to thioredoxin (Trx), inhibiting its antioxidant activity and promoting oxidative stress. Txnip is involved in signaling pathways like AMPK/mTOR and NF-κB, linking metabolism and inflammation.
Dysregulated Txnip expression correlates with diabetes, where it exacerbates pancreatic β-cell dysfunction. It also contributes to cancer progression and neurodegenerative diseases. Emerging research targets Txnip for therapeutic development, with small-molecule inhibitors under preclinical evaluation to modulate its pro-oxidant and pro-inflammatory effects.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

TXNIP Recombinant Monoclonal Antibody(CSB-RA949793A0HU)

Validated Data

Western Blot
Positive WB detected in: THP-1 whole cell lysate(30µg), K562 whole cell lysate(30µg)
All lanes: TXNIP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 44 kDa kDa
Observed band size: 53 kDa kDa
Exposure time：2min

IHC image of CSB-RA949793A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing K562 cells stained with CSB-RA949793A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

TXNIP Antibody(CSB-PA880966LA01HU)

Validated Data

Immunofluorescence staining of MCF-7 cells with CSB-PA880966LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Human Thioredoxin-interacting protein(TXNIP) ELISA kit(CSB-EL025383HU)

Validated Data

Code: CSB-EL025383HU

Size: 96T,5×96T,10×96T

Sensitivity: 3.9 pg/mL

Detection Range: 15.6 pg/mL-1000 pg/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following Txnip reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

Txnip Antibodies

Txnip Proteins

Txnip ELISA Kits

Txnip Antibodies

Txnip Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA976170	TXNIP Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA296101	TXNIP Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA880966LA01HU	TXNIP Antibody	Human	ELISA, IF
CSB-PA880966LB01HU	TXNIP Antibody, HRP conjugated	Human	ELISA
CSB-PA880966LC01HU	TXNIP Antibody, FITC conjugated	Human
CSB-PA880966LD01HU	TXNIP Antibody, Biotin conjugated	Human	ELISA
CSB-RA949793A0HU	TXNIP Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, FC

Txnip Antibodies for Mus musculus (Mouse)

Code	Product Name	Species Reactivity	Application
CSB-PA803849LA01MO	Txnip Antibody	Mouse	ELISA
CSB-PA803849LB01MO	Txnip Antibody, HRP conjugated	Mouse	ELISA
CSB-PA803849LC01MO	Txnip Antibody, FITC conjugated	Mouse
CSB-PA803849LD01MO	Txnip Antibody, Biotin conjugated	Mouse	ELISA

Txnip Proteins

Txnip Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP880966HU CSB-EP880966HU CSB-BP880966HU CSB-MP880966HU CSB-EP880966HU-B	Recombinant Human Thioredoxin-interacting protein (TXNIP)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-CF880966HU	Recombinant Thioredoxin-interacting protein (TXNIP)	in vitro E.coli expression system
CSB-CF880966HUa6	Recombinant Thioredoxin-interacting protein (TXNIP)	in vitro E.coli expression system
CSB-EP880966HU	Recombinant Human Thioredoxin-interacting protein (TXNIP),Escherichia Coli	E.coli
CSB-EP880966HUg5	Recombinant Human Thioredoxin-interacting protein (TXNIP)	E.coli

Txnip Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP683628RA CSB-EP683628RA CSB-BP683628RA CSB-MP683628RA CSB-EP683628RA-B	Recombinant Rat Thioredoxin-interacting protein (Txnip)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

Txnip Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP639475PI CSB-EP639475PI CSB-BP639475PI CSB-MP639475PI CSB-EP639475PI-B	Recombinant Pig Thioredoxin-interacting protein (TXNIP)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

Txnip Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

Code	Product Name	Source
CSB-YP732667PYX CSB-EP732667PYX CSB-BP732667PYX CSB-MP732667PYX CSB-EP732667PYX-B	Recombinant Pongo abelii Thioredoxin-interacting protein (TXNIP)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

Txnip Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP803849MO	Recombinant Mouse Thioredoxin-interacting protein (Txnip)	Yeast
CSB-EP803849MO	Recombinant Mouse Thioredoxin-interacting protein (Txnip)	E.coli
CSB-YP803849MO CSB-EP803849MO CSB-BP803849MO CSB-MP803849MO CSB-EP803849MO-B	Recombinant Mouse Thioredoxin-interacting protein (Txnip)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP803849MOc7	Recombinant Mouse Thioredoxin-interacting protein (Txnip)	E.coli
CSB-EP803849MO1	Recombinant Mouse Thioredoxin-interacting protein (Txnip), partial	E.coli
CSB-EP803849MOg5	Recombinant Mouse Thioredoxin-interacting protein (Txnip)	E.coli