PODXL encodes Podocalyxin-like protein. Commonly known as PCLP1, it's a type I transmembrane sialomucin. This protein functions by binding to ligands, modulating cell adhesion and migration. It's involved in the PI3K/Akt and Wnt/β-catenin signaling pathways, which play crucial roles in cell growth and survival.
Aberrant expression of PODXL is linked to various diseases, especially cancers such as renal cell carcinoma and breast cancer. In drug development, targeting PODXL is an emerging strategy. Some pre - clinical studies are exploring agents that can inhibit its function, aiming to block tumor progression and metastasis.
Popular
Product:
Recombinant Human Podocalyxin (PODXL), partial(CSB-YP518830HU)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.
PODXL Monoclonal Antibody(CSB-MA134471A0m)
Validated Data
Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PODXL antibody at 0.43µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
All lanes: PODXL antibody at 1.28µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200
Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200
Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating PODXL in HEK293 whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA134471A0m (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)
The following PODXL
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
