Recombinant Mouse Podocalyxin (Podxl), partial

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MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Podxl

Purity

>85% (SDS-PAGE)

Target Names

Podxl

Uniprot NO.

Q9R0M4

Species

Mus musculus (Mouse)

Source

Yeast

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
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Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Gene References into Functions

Podocalyxin-like protein 1 is a relevant marker for human c-kit(pos) cardiac stem cells.( PMID:23897803
Podxl-KO led to heightened G-CSF activation of Rap1a(GTP), and Rap1a(GTP) inhibition attenuated Podxl-KO neutrophil migration. Studies have revealed novel roles for Podxl as an important modulator of neutrophil and monocyte formation and of Rap1a activation during stress hematopoiesis. PMID:28408238
Podxl-overexpression in neural stem/progenitor cells leads to an up-regulation of Annexin A2. PMID:27470587
when endogenous podocalyxin was removed from highly metastatic 4T1 mammary tumor cells there was a decrease in collective invasion . Podocalyxin is a tumor cell-intrinsic regulator of experimental collective tumor cell invasion and tumor budding. PMID:26796961
Podocalyxin has a role in glioblastoma multiforme cell invasion and proliferation via beta-catenin signaling PMID:25349993
Loss of podocalyxin from the lung vasculature results in changes to the lung structure and function including increased lung volume when inflated under constant pressure (25 cm H2O) and changes to the matrix composition. PMID:25303643
CTGF is an important mediator of high glucose-induced podocyte damage and decreases the protein level of podocalyxin by the ERK1/2 pathway. PMID:21602138
platelet Podxl is involved in the control of hemostasis acting as a platelet co-stimulator, likely due to its pro-adhesive properties PMID:22016802
Full-length recombinant murine podocalyxin recruits NHERF-1 to the apical domain. Ectopic expression in human & canine epithelial cells leads to microvilli formation on an apical domain extending laterally to the junctional complexes. PMID:17311105
Podocalyxin plays multiple roles in neural development PMID:20706633
PODXL may act as a costimulator of agonists in the activation of platelets and formation of a stable thrombus. PMID:20223501
Data show that in podocalyxin-deficient mice, the thickness of the main carotid artery is significantly increased, and there is an increased number of choroidal capillaries lining the ventricular spaces. PMID:19837166
Podocalyxin mRNA was widely expressed at least from E14, the first age we studied, and expression remained high until adulthood PMID:15661640
In mice expressing an Erythropoietin receptor allele, compromised erythropoietin-induced podocalyxin expression correlated with enucleated red cells in bone marrow. PMID:17403918
PODXL(hi)/CD49f(hi) MSCs were less prone to produce lethal pulmonary emboli PMID:18818395
Podocalyxin selectively marks erythroid-committed progenitors during anemic stress but is dispensable for efficient recovery. PMID:19004540
Podocalyxin is required for normal kidney function PMID:11435469

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Subcellular Location

Apical cell membrane. Cell projection, microvillus. Membrane raft. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Membrane; Single-pass type I membrane protein.

Protein Families

Podocalyxin family

Tissue Specificity

Expressed in liver cells and hematopoietic cells (at protein level). Glomerular epithelium cell (podocyte).

Database Links

UNIGENE: Mm.89918

KEGG: mmu:27205

STRING: 10090.ENSMUSP00000026698

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