L1CAM

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized L1CAM at 2 μg/ml can bind Anti-L1CAM Rabbit Monoclonal Antibody (CSB-RA595071A0HU), the EC₅₀ is 5.384-9.380 ng/ml.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Mouse L1cam at 2 μg/ml can bind Anti-L1CAM recombinant antibody(CSB-RA012704MA1HU). The EC₅₀ is 1.827-2.141 ng/mL.

The purity of L1cam was greater than 95% as determined by SEC-HPLC

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis L1CAM at 2 μg/ml can bind Anti-L1CAM recombinant antibody(CSB-RA012704MA1HU). The EC₅₀ is 1.496-1.709 ng/mL.

The purity of L1CAM was greater than 95% as determined by SEC-HPLC

L1CAM Recombinant Monoclonal Antibody

Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate
All lanes: L1CAM antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 141, 140, 139 kDa
Observed band size: 90 kDa

Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA07439A0Rb at dilution of 1:100

Immunofluorescent analysis of A549 cells using CSB-PA07439A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)