CD40, Tumor Necrosis Factor Receptor Superfamily Member 5 (TNFRSF5), alias Bp50, CD40L, etc. CD40 is encoded by the human CD40 gene and is widely expressed in immune cells, especially B cells, dendritic cells (DCs), and monocytes. CD40 is a single-chained transmembrane protein comprising an extracellular domain, a transmembrane region, and a cytoplasmic region. The extracellular domain binds to its ligand CD40L (CD40 ligand) and triggers signaling.
CD40 plays an important role in linking innate and adaptive immunity, and its functions extend to B-cell activation, antigen presentation, and inflammation modulation. CD40 is important for humoral immune response, immune memory formation, and immune regulation. CD40 signaling has also been extensively studied in immunotherapy research. has also been extensively studied in immunotherapy research to develop new therapeutic approaches to enhance or inhibit specific responses of the immune system.
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Product:
Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active)(CSB-MP004936HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 μg/ml can bind CD40L (CSB-MP004937HU3), the EC50 is 3.112-3.858 ng/ml.
Activity
Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay.
The purity of CD40 was greater than 95% as determined by SEC-HPLC
CD40 Recombinant Monoclonal Antibody(CSB-RA004936MA1HU)
Validated Data
Immunofluorescence staining of A549 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
Immunofluorescence staining of PC-3 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
Overlay Peak curve showing THP-1 cells surface stained with CSB-RA004936MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,012 events was performed.
The following CD40
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
