Abbreviation
Recombinant Human TNFRSF17 protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of >2.5x104 IU/mg in the presence of 100 ng/ml human APRIL.
Alternative Names
B cell maturation antigen; B cell maturation factor; B cell maturation protein; B-cell maturation protein; BCM; BCMA; CD269; CD269 antigen; TNFRSF17; TNR17_HUMAN; Tumor necrosis factor receptor superfamily member 17
Species
Homo sapiens (Human)
Target Protein Sequence
AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 30 % acetonitrile, 0.1 % TFA
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
TNFRSF17 (BCMA) serves as a critical receptor for APRIL and BAFF, making it a key therapeutic target in multiple myeloma and B-cell malignancies. This tag-free recombinant human BCMA fragment, spanning residues 5–54 of the extracellular ligand-binding domain, demonstrates confirmed biological activity with an ED50 no greater than 40 ng/ml in inhibiting APRIL-mediated proliferation of anti-IgM-stimulated murine B cells, corresponding to a specific activity exceeding 2.5 × 10⁴ IU/mg. This functional validation supports use in competitive inhibition assays, blocking antibody screening, therapeutic antibody epitope mapping, and ligand-binding interaction studies via ELISA, SPR, or BLI. Expressed in *E. coli* to yield a compact, properly folded domain at >98% purity with endotoxin levels below 1.0 EU/μg, this preparation satisfies the purity and endotoxin criteria typical for cell-based bioassays and drug discovery screening of biologic or small-molecule inhibitors targeting the BCMA–APRIL axis.
