Abbreviation
Recombinant Human TNFSF9 protein, partial (Active)
Purity
>95% as determined by SDSPAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by stimulation of IL?8 production using human PBMC is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Alternative Names
4 1BB L; 4 1BB ligand; 4 1BBL; 4-1BB ligand; 4-1BBL; Cd137l; Cd157l; Homolog of mouse 4 1BB L; Homolog of mouse 4 1BBL; ILA ligand (TNF related); Ly63l; Receptor 4 1BB ligand; TNF superfamily member 9; TNFL9_HUMAN; Tnfsf9; TNLG5A; Tumor necrosis factor (ligand) superfamily member 9; Tumor necrosis factor ligand 5A; Tumor necrosis factor ligand superfamily member 9; Tumor necrosis factor superfamily member 9
Species
Homo sapiens (Human)
Expression Region
71-254aa
Target Protein Sequence
REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
TNFSF9 (4-1BB ligand) plays a central role in T-cell co-stimulation and anti-tumor immunity, making it a critical reagent for immuno-oncology research. This tag-free recombinant human TNFSF9 (aa 71–254), expressed in *E. coli*, demonstrates potent bioactivity with an ED50 of less than 10 ng/mL in IL-8 stimulation of human PBMCs, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg—providing reliable performance in PBMC activation assays, NF-κB signaling pathway studies, and T-cell co-stimulation experiments. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that would otherwise confound cytokine readouts in sensitive immune cell functional assays. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, supports use as a reference standard in cytokine detection assays, a positive control for antibody validation, and a functional ligand in tumor biology animal models.
