Recombinant Human Psychosine receptor (GPR65)

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Code: CSB-CF810304HU

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US$1620

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Express system: in vitro E.coli expression system

Species: Homo sapiens (Human)

Tag Info: N-terminal 10xHis-tagged

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Target Background

Product Details

Abbreviation

Recombinant Human GPR65 protein

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

GPR65

Uniprot NO.

Q8IYL9

Research Area

Cancer

Species

Homo sapiens (Human)

Source

in vitro E.coli expression system

Expression Region

1-337aa

Target Protein Sequence

MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAKKESELGIYLFSLSLSDLLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSSTAFLTCIAVDRYLAVVYPLKFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYCDAEKSNFTLCYDKYPLEKWQINLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATENKEKKRIIKLLVSITVTFVLCFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

40.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

Please contact us to get it.

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Target Background

Function(From Uniprot)

Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance. May have a role in activation-induced cell death or differentiation of T-cells.

Gene References into Functions

TDAG8 functions as a contextual tumor suppressor down-regulated in hematological malignancies. PMID:29017562
Study results suggest greater T cell death associated gene-8 (TDAG8) expression in patients with panic disorder compared to healthy subjects, and directly link TDAG8 expression and the severity of the panic disorder symptoms PMID:28736033
this study establishes a role for GPR65 in inflammatory bowel disease susceptibility and identify lysosomal dysfunction as a potentially causative element in pathogenesis PMID:27287411
TDAG8 expression is decreased by more than 50%. PMID:24152439
GPR65 is a regulator upstream of MMP3, which is regulated by proton-sensing G-protein-coupled receptors PMID:23707809
Acidosis promotes Bcl-2 family-mediated evasion of apoptosis: involvement of acid-sensing G protein-coupled receptor Gpr65 signaling to Mek/Erk. PMID:22685289
Data suggest that one physiological function of TDAG8 is negative regulation of inflammation by inhibiting production of pro-inflammatory cytokines in T-lymphocytes and macrophages. PMID:22445881
These results support the hypothesis that TDAG8 enhances tumor development by promoting adaptation to the acidic environment to enhance cell survival/proliferation. PMID:20855608
GPR4 and TDAG8 overexpression in human tumors plays a role in driving or maintaining tumor formation PMID:15221007
TDAG8 is one of the proton-sensing GPCRs coupling to adenylyl cyclase and psychosine, and its related lysosphingolipids behave as if they were antagonists against protein-sensing receptors, including TDAG8, GPR4, and OGR1. PMID:15326175
TDAG8 may play biological roles in immune response and cellular transformation under conditions accompanying tissue acidosis. PMID:15618224
Expression of TDAG8 by immune cells may regulate responses in acidic microenvironments. PMID:15665078
At least partly, TDGA8 mediates extracellular acidification-induced inhibition of proinflammatory cytokine production through the Gs protein/cyclic AMP-dependent protein kinase A signaling pathway in transgenic mouse macrophages. PMID:19234222