Abbreviation
Recombinant Human CCR4 protein (Active)
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/mL can bind Anti-CCR4 recombinant antibody (CSB-RA004843MA01HU). The EC50 is 2.803-3.369 ng/mL.
②Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) with an affinity constant of 3.16 nM as detected by SPR Assay(Biacore T200).
③Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12)(WeSPRTM200) .
Alternative Names
C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
1-360aa
Target Protein Sequence
MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVFVFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAADQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRARTLTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKVLSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVLFLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLNPIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQSTMDHDLHDAL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
PBS, 0.05% FOS-12, pH 8.0 6% Trehalose
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/ml can bind Anti-CCR4 recombinant antibody (CSB-RA004843MA01HU). The EC50 is 2.803-3.369 ng/mL.
-
Activity
Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) with an affinity constant of 3.16 nM as detected by SPR Assay(Biacore T200).
-
Activity
Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12)(WeSPRTM 200) .
Description
CCR4 is a seven-transmembrane GPCR notoriously difficult to produce as a full-length construct, and this recombinant human CCR4 (1–360 aa) overcomes that challenge through a cell-free E. coli expression system that enables recovery of the complete receptor sequence, including all transmembrane domains, with an N-terminal 10×His tag for oriented capture. Conformational integrity is supported by SPR data demonstrating high-affinity binding to an anti-CCR4 monoclonal antibody, with KD values of 3.16 nM (Biacore T200) and 1.71 nM (MetaSPR in FOS-12 detergent), while functional ELISA confirms dose-dependent interaction with an EC50 of 2.80–3.37 ng/mL. These validated binding characteristics provide a suitable basis for therapeutic antibody epitope mapping, competitive blocking antibody screening, affinity characterization by SPR or BLI, and small-molecule inhibitor discovery campaigns targeting CCR4-mediated signaling. Purity exceeds 85% by SDS-PAGE, which meets the quality thresholds commonly required for use as a positive control in receptor-ligand interaction assays.
