Abbreviation
Recombinant Human CCR1 protein-VLPs (Active)
Purity
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CCR1 at 10 μg/mL can bind Anti-CCR1 recombinant antibody(CSB-RA004839MA1HU). The EC50 is 9.690-10.94 ng/mL.The VLPs (CSB-MP3838) is negative control.
Alternative Names
C-C chemokine receptor type 1; C-C CKR-1; CC-CKR-1; CCR-1; CCR1; HM145; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor (MIP-1alpha-R); RANTES-R; CD191; CCR1; CMKBR1, CMKR1, SCYAR1
Species
Homo sapiens (Human)
Expression Region
1-355aa
Target Protein Sequence
METPNTTEDYDTTTEFDYGDATPCQKVNERAFGAQLLPPLYSLVFVIGLVGNILVVLVLVQYKRLKNMTSIYLLNLAISDLLFLFTLPFWIDYKLKDDWVFGDAMCKILSGFYYTGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGVITSIIIWALAILASMPGLYFSKTQWEFTHHTCSLHFPHESLREWKLFQALKLNLFGLVLPLLVMIICYTGIIKILLRRPNEKKSKAVRLIFVIMIIFFLFWTPYNLTILISVFQDFLFTHECEQSRHLDLAVQVTEVIAYTHCCVNPVIYAFVGERFRKYLRQLFHRRVAVHLVKWLPFLSVDRLERVSSTSPSTGEHELSAGF
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
CSB-MP004839HU is detected by Mouse anti-6*His monoclonal antibody.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CCR1 at 10 μg/ml can bind Anti-CCR1 recombinant antibody(CSB-RA004839MA1HU). The EC50 is 9.690-10.94 ng/mL.The VLPs (CSB-MP3838) is negative control.
-
The purity of VLPs was greater than 90% as determined by SEC-HPLC
Description
CCR1 mediates chemotactic responses to inflammatory chemokines including CCL3 (MIP-1α) and CCL5 (RANTES), making it a target of interest in cancer immunology and inflammatory disease research. This full-length receptor (aa 1–355) is presented in virus-like particles produced in mammalian cells, a format that preserves native membrane topology and post-translational modifications critical for conformational integrity of seven-transmembrane receptors. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 9.69–10.94 ng/mL, confirming that the extracellular epitopes retain their native structure and supporting use in therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody validation assays. With endotoxin levels below 1.0 EU/μg, this VLP-displayed receptor meets the quality thresholds commonly required for ligand-binding studies, receptor-ligand interaction characterization by surface plasmon resonance or biolayer interferometry, and small-molecule inhibitor screening campaigns targeting chemokine receptor pathways in oncology research.
