Home
Product
Protein
Transmembrane Proteins
Recombinant Escherichia coli Aquaporin Z (aqpZ),Nanodisc

Recombinant Escherichia coli Aquaporin Z (aqpZ),Nanodisc

CSB-CF352724ENV-N

Rate （N/A）
Review （0）

Size:

20ug100ug

US$1820

Quantity:

Express system: in vitro E.coli expression system

Species: Escherichia coli (strain K12)

Tag Info: N-terminal 6xHis-tagged

Send an Inquiry

Start an on-line Chat

Orders can only be placed from within the U.S.

Promotion

Transmembrane Protein Questionnaire with Gifts

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

aqpZ

Uniprot NO.

P60844

Research Area

Microbiology

Species

Escherichia coli (strain K12)

Source

in vitro E.coli expression system

Expression Region

1-231aa

Target Protein Sequence

MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD

Mol. Weight

27.7 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

Please contact us to get it.

Images

Recombinant Escherichia coli Aquaporin Z (aqpZ),Nanodisc

Description

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function

Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.

Gene References into Functions

Induction of an immune response against AqpZ or its homologous regions can also trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system. PMID:23008451
Data show that the open and closed states of AqpZ directly relate to the side chain conformation of residue R189. PMID:21315687
the 2.5 A resolution structure of AqpZ suggests aquaporin selectivity results both from a steric mechanism due to pore size and from specific amino acid substitutions that regulate the preference for a hydrophobic or hydrophilic substrate PMID:14691544
the difference in Arg-189 displacements is correlated with a strong electron density between first transmembrane helices of two open channels; the observed Arg-189 conformations are stabilized by asymmetrical subunit interactions in tAqpZ PMID:16239219
analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities PMID:16399837
biochemical analysis of permeability and solute transport characteristics of amphiphilic triblock-polymer vesicles containing the bacterial water-channel protein Aquaporin Z PMID:18077364