WARS1 Recombinant Monoclonal Antibody

Code: CSB-RA293319A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P23381

Target Names

WARS1

Alternative Names

Gamma 2 antibody; GAMMA-2 antibody; Gamma2 antibody; hWRS antibody; IFI 53 antibody; IFI53 antibody; IFP 53 antibody; IFP53 antibody; Interferon induced protein 53 antibody; Interferon-induced protein 53 antibody; SYWC_HUMAN antibody; T2-TrpRS antibody; TrpRS antibody; Tryptophan tRNA ligase; cytoplasmic antibody; Tryptophan tRNA ligase 1 cytoplasmic antibody; Tryptophan tRNA ligase antibody; Tryptophan--tRNA ligase antibody; Tryptophanyl tRNA synthetase antibody; Tryptophanyl tRNA synthetase cytoplasmic antibody; WARS antibody; WARS protein antibody; WRS antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human WARS1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: A-431 whole cell lysate(30µg), HeLa whole cell lysate, HL-60 whole cell lysate(30µg)
All lanes: WARS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3min
Overlay Peak curve showing HepG2 cells stained with CSB-RA293319A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

WARS1, also known as tryptophanyl-tRNA synthetase, plays an essential role in protein biosynthesis by catalyzing the attachment of tryptophan to its cognate tRNA. Beyond its canonical function in translation, WARS1 has garnered significant research interest for its non-canonical activities, particularly as an interferon-induced protein with roles in angiogenesis regulation and immune signaling. The protein can be cleaved to generate fragments with distinct biological functions, making it a compelling target for studies spanning cancer biology, vascular research, and inflammatory responses.

This recombinant monoclonal antibody, clone 8D4, offers the consistency and reproducibility that demanding research workflows require. Developed using recombinant technology in a rabbit host, it provides sequence-defined specificity against human WARS1, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The antibody is raised against a synthetic peptide derived from human WARS1 and purified through affinity chromatography to ensure high purity.

Validation studies demonstrate reliable performance across multiple applications. In western blot analysis, the antibody detects a band at the predicted molecular weight of 53 kDa in A-431, HeLa, and HL-60 whole cell lysates, confirming specificity across diverse human cell types. Flow cytometry validation using HepG2 cells shows clear separation between antibody-stained and control populations, supporting intracellular detection applications.

With validated performance in ELISA, western blot, and flow cytometry, this antibody provides flexibility for researchers investigating WARS1 in contexts ranging from interferon-mediated responses to aminoacyl-tRNA synthetase biology and tumor microenvironment studies.

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Target Background

Function(From Uniprot)

Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.

Gene References into Functions

Based on these results, secretion of full-length tryptophanyl-tRNA synthetase appears to work as a primary defence system against infection, acting before full activation of innate immunity. PMID:27748732
findings establish WARS as a gene whose mutations may cause distal hereditary motor neuropathy and alter canonical and non-canonical functions of tryptophanyl-tRNA synthetase. PMID:28369220
Overexpression of WARS predicts no recurrence and good survival for triple-negative breast cancer patients. PMID:26209610
Tryptophanyl-tRNA synthetase expression is up-regulated in patients with rheumatoid arthritis. PMID:24515434
Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14) PMID:23670221
Indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase may play critical roles in the immune pathogenesis of chronic kidney disease. PMID:23651343
Naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. PMID:21442253
Tryptophanyl-tRNA synthetase down-regulation by hypoxia may be a factor responsible for low TrpRS in pancreatic tumors with high metastatic ability. PMID:21926542
Mini-tryptophanyl-tRNA synthetase inhibited ischemic angiogenesis in rats. PMID:20963594
Tryptophanyl-tRNA synthetase is a multidomain protein exhibiting excellent allosteric communication, and this research has provided valuable structural as well as functional insights into the protein. PMID:19768679
Low tryptophanyl-tRNA synthetase is associated with recurrence in colorectal cancer. PMID:19900940
In this study, we show that a recombinant form of a COOH-terminal fragment of TrpRS is a potent antagonist of vascular endothelial growth factor-induced angiogenesis in a mouse model and of naturally occurring retinal angiogenesis in the neonatal mouse PMID:11773625
Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS PMID:11773626
Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains PMID:11834741
The recently discovered antiangiogenic and cell-signaling activities of tryptophanyl-tRNA synthetase bioactive fragments are discussed in this review. PMID:12416978
TrpRS may have a role in the maintenance of vascular homeostasis PMID:14630953
results suggest that mammalian and bacterial tryptophanyl-tRNA synthetase might use different mechanisms to recognize the substrate and modeling studies indicate that transfer RNA binds with the dimeric enzyme PMID:14660560
A crystal structure of human tryptophanyl-tRNA synthetase was solved at 2.1 A with a tryptophanyl-adenylate bound at the active site PMID:14671330
May play an important role in the intracellular regulation of protein synthesis under conditions of oxidative stress. PMID:15628863
These crystals captured two conformations of the human tryptophanyl-tRNA synthetase and tRNATrp complex, which are nearly identical with respect to the protein and a bound tryptophan. PMID:16724112
The first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. PMID:16798914
Results provide the first evidence of the involvement of heme in regulation of TrpRS aminoacylation activity. PMID:17877375
the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS. PMID:17999956
Analysis of the molecular basis of the mechanisms of the substrate recognition and the activation reaction by tryptophanyl-tRNA synthetase. PMID:18180246
Indoleamine 2,3-dioxygenase (IDO)-expression in antigen-presenting cells (APCs) may control autoimmune responses by depleting the available tryptophan, whereas tryptophanyl-tRNA synthetase (TTS) may counteract this effect PMID:19363598