VSIR Recombinant Monoclonal Antibody

Code: CSB-RA884484MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA

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Target Background

Product Details

Uniprot NO.

Q9H7M9

Target Names

VSIR

Alternative Names

B7 H5 antibody; B7H5 antibody; C10orf54 antibody; chromosome 10 open reading frame 54 antibody; DD1alpha antibody; GI24 antibody; GI24_HUMAN antibody; PD-1H antibody; PDCD1 homolog antibody; Platelet receptor Gi24 antibody; PP2135 antibody; sisp 1 antibody; SISP1 antibody; stress induced secreted protein 1 antibody; UNQ730/PRO1412 antibody; V domain Ig suppressor of T cell activation antibody; V set domain containing immunoregulatory receptor antibody; V set immunoregulatory receptor antibody; V type immunoglobulin domain containing suppressor of T cell activation antibody; VISTA antibody

Species Reactivity

Human

Immunogen

Recombinant Human VSIR protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

15F4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Recommended Dilution

Application	Recommended Dilution
ELISA	1:5000-1:50000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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The Binding Activity of Human VSIR with Anti-VSIR Recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human VSIR (CSB-MP884484HU) at 2 μg/mL can bind Anti-VSIR recombinant antibody. The EC₅₀ is 20.84-22.73 ng/mL.

Description

VSIR, also known as VISTA (V-domain Ig Suppressor of T cell Activation), represents a critical immune checkpoint regulator that has emerged as a significant target in immunology and oncology research. This inhibitory receptor, expressed predominantly on myeloid cells and T lymphocytes, plays a central role in suppressing T cell activation and maintaining immune homeostasis. Understanding VSIR biology has become increasingly important for researchers investigating immune regulation, tumor microenvironment dynamics, and potential therapeutic interventions targeting checkpoint pathways.

This recombinant monoclonal antibody, clone 15F4, offers the consistency and reliability that demanding experimental workflows require. Because it is produced using recombinant technology with a defined sequence, you can expect exceptional lot-to-lot reproducibility throughout your research program. The human IgG1 isotype format and affinity-chromatography purification ensure high purity and specificity for detecting human VSIR.

Functional validation demonstrates robust binding activity, with the antibody showing strong interaction with immobilized human VSIR protein in ELISA format. The EC50 values of 20.84–22.73 ng/mL reflect excellent sensitivity, allowing you to work across a broad dilution range of 1:5000 to 1:50000 depending on your specific assay conditions. This flexibility enables optimization for various experimental setups while maintaining reliable detection.

The unconjugated format provides versatility for pairing with your preferred secondary detection systems. Supplied in a glycerol-based PBS buffer with Proclin 300 preservative, the antibody maintains stability when stored at -20°C or -80°C. For researchers exploring immune checkpoint biology, T cell regulation, or cardiovascular-related immune mechanisms, this antibody delivers the reproducible performance essential for generating publication-quality data.

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Target Background

Function(From Uniprot)

Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.

Gene References into Functions

Data indicate that VISTA is predominantly expressed and up-regulated in the high-density infiltrated immune cells but minimal in pancreatic cancerous cells. PMID:29771768
High VISTA expression is associated with colorectal carcinoma. PMID:30128738
VISTA expression supports immune-complex inflammation in collagen antibody-induced arthritis and VISTA is expressed in human synovium PMID:29216931
This study is the first to describe the expression of VISTA-expressing lymphocytes in melanoma samples and in the context of acquired resistance to immune checkpoint inhibitors PMID:28776578
Taken together, the results indicated that the VISTA high and CD8 low group, as an immunosuppressive subgroup, might be associated with a poor prognosis in primary OSCC. These findings indicated that VISTA might be a potential immunotherapeutic target in OSCC treatment. PMID:28236118
this review describes the functions of VISTA in the context of cancer immunotherapy PMID:28258694
overexpression of the newly described co-stimulatory molecule, PD1 homologue (PD-1H) in human monocyte/macrophages is sufficient to induce spontaneous secretion of multiple cytokines. PMID:25279955
p53-induced expression of DD1alpha prevents persistence of cell corpses and ensures efficient generation of precise immune responses in mice. PMID:26228159
Results suggest that GI24 contributes to tumor-invasive growth in the collagen matrix by augmenting cell surface MT1-MMP. PMID:20666777

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta

Database Links

HGNC: 30085

UNIGENE: Hs.47382

KEGG: hsa:64115

STRING: 9606.ENSP00000378409

OMIM: 615608

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