TPBG Recombinant Monoclonal Antibody

Code: CSB-RA024093MA2HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

Q13641

Target Names

TPBG

Alternative Names

Trophoblast glycoprotein antibody; 5T4 oncofetal antigen antibody; 5T4 oncofetal trophoblast glycoprotein (5T4 oncotrophoblast glycoprotein) antibody; M6P1 antibody; Wnt-activated inhibitory factor 1 (WAIF1) antibody; TPBG antibody; 5T4

Species Reactivity

Human

Immunogen

Recombinant Human TPBG protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

9H9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing MCF7 cells stained with CSB-RA024093MA2HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

TPBG, also known as 5T4 oncofetal antigen, is a leucine-rich repeat glycoprotein that plays a significant role in embryonic development and cancer biology. Originally identified in trophoblast cells, TPBG has gained considerable attention as a tumor-associated antigen due to its restricted expression in normal adult tissues but frequent overexpression across multiple carcinoma types. The protein functions as a Wnt-activated inhibitory factor, positioning it at the intersection of developmental signaling pathways and oncogenic processes, making it a compelling target for both basic stem cell research and translational oncology studies.

This recombinant monoclonal antibody, clone 9H9, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly with a human IgG1 framework, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived reagents. The antibody was raised against recombinant human TPBG protein and demonstrates specificity for human samples.

Validation studies confirm robust performance in flow cytometry applications, with recommended dilutions ranging from 1:50 to 1:200. Testing on MCF7 cells, a human breast adenocarcinoma line known to express TPBG, demonstrated clear positive signal separation from isotype control, indicating specific detection of surface TPBG expression. The antibody is also validated for ELISA applications, providing flexibility for researchers working across different experimental platforms.

For investigators exploring stem cell biology, tumor immunology, or developing therapeutic strategies targeting oncofetal antigens, this antibody provides a dependable tool for characterizing TPBG expression patterns in human samples.

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Target Background

Function(From Uniprot)

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Gene References into Functions

In extravillous trophoblasts, 5T4 may function in epithelial-to-mesenchymal transition during placentation. The role of syncytiotrophoblast 5T4 is unknown, but its abundance in shed syncytial vesicles may signify route of sensitization of the maternal immune system. PMID:28481180
Collectively, these findings demonstrate that an anti-5T4 antibody-drug conjugate reduces the fraction of cancer stem cells (CSC), and prevents local recurrence and suggest a novel therapeutic approach for patients with head and neck squamous cell carcinoma. PMID:27780858
We obtained GWS evidence (P < 2.7 x 10(-6)) for gene-based association in the total sample with a novel locus, TPBG (P = 1.8 x 10(-6)). PMID:28183528
In conclusion, the present study provided evidence that TPBG is involved in PDAC metastasis, and that TPBG and its associated signaling pathways may be a suitable target for pancreatic ductal adenocarcinoma therapy. PMID:25738465
5T4 expression is mechanistically associated with the directional movement of cells through epithelial mesenchymal transition, facilitation of CXCL12/CXCR4 chemotaxis, blocking of canonical Wnt/beta-catenin while favouring non-canonical pathway signalling PMID:25066861
Tyrosine 325 plus the leucine-rich repeat 1 surface centered on a second exposed aromatic residue, phenylalanine 97, are essential for inhibition of Wnt/beta-catenin signaling. PMID:24582434
The surface expression of 5T4 marks the use of the CXCR4 rather than the CXCR7 receptor, with distinct consequences for CXCL12 exposure. PMID:22956548
5T4 is a potential new antigen for targeted therapies such as immunotherapy in MPM, as it is overexpressed on mesothelioma cells and recognised by 5T4-specific cytotoxic T-cells. PMID:22498111
Glycosylation and epitope mapping of the 5T4 glycoprotein oncofoetal antigen PMID:11903056
Results sugest that 5T4 is a transient marker of human embryonic stem cell differentiation & that 5T4 phenotype, colony seeding density and culture conditions influence the maintenance of pluripotent hES cells and their differentiation to neural lineages. PMID:16616918
there is a repertoire of CD8 T cell recognition of 5T4 in normal human donors and some candidate HLA-A*0201 epitopes have been identified PMID:16646078
the mRNA expression of trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia. PMID:17978129
5T4-based cancer vaccine: cytotoxic T lymphocyte epitopes identified in Trovax-vaccinated colorectal cancer patients PMID:18567615
colorectal cancer patients who had preexisting cell proliferative responses to 5T4 were longer-term survivors PMID:18833005

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Database Links

HGNC: 12004

UNIGENE: Hs.71947

KEGG: hsa:7162

STRING: 9606.ENSP00000358765

OMIM: 190920

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