PRKCSH Recombinant Monoclonal Antibody

Code: CSB-RA439154A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

P14314

Target Names

PRKCSH

Alternative Names

80K-H protein antibody; AGE-binding receptor 2 antibody; AGE-R2 antibody; G19P1 antibody; GLU2B_HUMAN antibody; Glucosidase 2 subunit beta antibody; Glucosidase II beta subunit antibody; Glucosidase II subunit beta antibody; Hepatocystin antibody; PCLD antibody; PKCSH antibody; PLD1 antibody; PRKCSH antibody; Protein kinase C substrate 60.1 kDa protein heavy chain antibody; Protein kinase C substrate 80 Kda protein antibody; Protein kinase C substrate 80K-H antibody; Protein kinase C substrate; 80 Kda protein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PRKCSH protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7F11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

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Images

Overlay Peak curve showing 786-O cells stained with CSB-RA439154A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

PRKCSH, also known as glucosidase II subunit beta or hepatocystin, serves as an essential component of the endoplasmic reticulum quality control machinery. This protein plays a critical role in the processing of newly synthesized glycoproteins by functioning as the regulatory beta subunit of glucosidase II, which trims glucose residues from N-linked oligosaccharides during protein folding. Beyond its glycan processing function, PRKCSH has garnered significant research attention due to its involvement in polycystic liver disease and its identification as an advanced glycation end product receptor. These diverse biological roles make PRKCSH a valuable target for researchers investigating ER stress responses, protein quality control mechanisms, and hepatic cyst formation.

This recombinant monoclonal antibody, clone 7F11, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human PRKCSH, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format and affinity-chromatography purification further contribute to reliable performance across experiments.

Validation studies confirm robust detection of PRKCSH in human samples using flow cytometry applications. Testing in 786-O renal carcinoma cells demonstrated clear positive signal separation from isotype control, with recommended working dilutions of 1:50 to 1:200 for flow cytometry analysis. The antibody is also validated for ELISA applications, providing flexibility for researchers employing multiple detection platforms.

This antibody supports investigations into cell marker expression and protein tagging studies, making it particularly suitable for researchers exploring ER-resident protein dynamics and glycoprotein processing pathways.

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Target Background

Function(From Uniprot)

Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.

Gene References into Functions

Results suggest that loss of PRKCSH and SEC63 leads to general defects in ciliogenesis, while quenching of the Wnt signaling cascade is cholangiocyte-restricted. PMID:28973524
This study demonstrated that Large copy number variations on germline level are not present in patients with a clinical diagnosis of Severe Polycystic Liver Disease. PMID:26365003
Polycystic liver disease is recessive at the cellular level, and loss of functional PRKCSH is an important step in cystogenesis. PMID:21856269
The induction of autophagy by hepatocystin deficiency is mediated through mammalian target of rapamycin (mTOR). PMID:21681021
Results provide evidence that mutations at the coding PRKCSH GAG repeat are a target of MSI and are selectively associated with the MSI-H phenotype in gastric carcinomas. PMID:21371016
The common SNPs tested in DDOST, PRKCSH and LGALS3 do not seem to be associated with diabetic micro- or macrovascular complications or with type 1 diabetes in Finnish patients. PMID:20490454
identified a total of 26 novel mutations in PRKCSH (n = 14) and SEC63 (n = 12), including four splice site mutations, eight insertions/ deletions, six non-sense mutations, and eight missense mutations PMID:20095989
Our results suggest that PRKCSH gene is not a major genetic cause of PCLD and there may be at least another locus responsible for the disease in Taiwan. PMID:19308730
PRKCSH functions as a chaperone-like molecule, which prevents endoplasmic reticulum-associated degradation of TRPP2. PMID:19801576
Mutations in this protein cause isolated autosomal dominant polycystic liver disease. PMID:12529853
germline mutations in PRKCSH as the probable cause of autosomal dominant polycystic liver disease PMID:12577059
autosomal dominant polycystic liver disease is genetically heterogeneous PMID:15057895
role of hepatocystin in carbohydrate processing and quality control of newly synthesized glycoproteins in the endoplasmic reticulum PMID:15188177
results identify 80K-H as a new player involved in GLUT4 vesicle transport and identify a link between a kinase involved in the insulin signalling cascade, PKCzeta, and a known component of the GLUT4 vesicle trafficking pathway, munc18c PMID:15707389
the majority of cysts from PRKCSH mutation carriers did not express hepatocystin PMID:18224332
Hepatocystin is not secreted in liver cyst fluids of autosomal dominant polycystic liver disease patients, suggesting that mutant hepatocystin is either not produced or degraded intracellularly. PMID:18419150
80K-H is a novel regulator of IP3R1 activity, and it may contribute to neuronal functions. PMID:18990696
These results indicate that insulin induces dynamic associations between PKCzeta, 80K-H, and munc18c and that 80K-H may act as a key signaling link between PKCzeta and munc18c in live cells. PMID:19061073