PRKCE Recombinant Monoclonal Antibody

Code: CSB-RA059812A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q02156

Target Names

PRKCE

Alternative Names

Protein kinase C epsilon type (EC 2.7.11.13) (nPKC-epsilon), PRKCE, PKCE

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PRKCE protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9F8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30µg), U-87MG whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Hela whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: PKC epsilon polymerase beta antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 85 kDa
Observed band size: 70-75 kDa
Exposure time：10s
Immunofluorescence staining of MCF-7 cell with CSB-RA059812A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Raji cells stained with CSB-RA059812A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Protein kinase C epsilon (PKCε) is a calcium-independent serine/threonine kinase that plays pivotal roles in cellular signaling pathways governing cell survival, proliferation, and migration. As a member of the novel PKC subfamily, PKCε has garnered significant research attention for its involvement in cardioprotection, neuroprotection, and its emerging role in cancer biology, where it often functions as an oncogene promoting tumor progression and therapeutic resistance.

This recombinant monoclonal antibody, clone 9F8, offers the reproducibility and consistency that demanding research applications require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies.

Validation across multiple platforms demonstrates the versatility of this reagent. Western blot analysis confirms robust detection in human cell lines including SH-SY5Y neuroblastoma, U-87MG glioblastoma, HEK293, and HeLa cells, with cross-species reactivity verified in mouse and rat brain tissue lysates. The observed band at 70-75 kDa, slightly lower than the predicted 85 kDa molecular weight, likely reflects post-translational processing or the specific epitope region detected. Immunofluorescence staining in MCF-7 cells reveals clear cytoplasmic localization patterns, while flow cytometry analysis using Raji cells demonstrates distinct positive population shifts compared to isotype controls.

Whether investigating PKCε signaling in neurological disease models, cardiac ischemia-reperfusion studies, or cancer research, this antibody provides a reliable tool with validated performance across western blotting, immunofluorescence, and flow cytometry workflows, supporting diverse experimental approaches within your research program.

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Target Background

Function(From Uniprot)

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1. Phosphorylates NLRP5/MATER and may thereby modulate AKT pathway activation in cumulus cells.

Gene References into Functions

PKCvarepsilon directly modulates the Aurora B-dependent abscission checkpoint by phosphorylating Aurora B at S227. This phosphorylation invokes a switch in Aurora B specificity, with increased phosphorylation of a subset of target substrates, including the CPC subunit Borealin. PMID:28004745
Results indicate an important role of protein kinase C epsilon (PKCepsilon) in autophagy and as a therapeutic target. PMID:29439667
PKC epsilon, activated by DCP-LA, increases elastin and fibulin-5/DANCE in the extracellular space of cultured fibroblasts by the mechanism independent of transcriptional and translational modulation or inhibition of elastolysis. PMID:29590645
The downregulation of mi143 promoted cell proliferation by regulating PKCepsilon in the hepatocellular carcinoma cells. PMID:28765889
These results demonstrate the relevance of hepatic diacylglycerol-induced PKCepsilon activation in the pathogenesis of NAFLD-associated hepatic insulin resistance in humans. PMID:28591572
Data show that protein kinase C epsilon (PKCepsilon) phosphorylates migration and invasion inhibitory protein (MIIP) at Ser303 and promotes its binding to RelA/p65, facilitating colorectal cancer metastasis. PMID:29038521
NF-kappaB signaling thus appears to play a critical role in promoting both castration resistance and enzalutamide resistance in PKC/Twist1 signaling in prostate cancer. PMID:28223364
results imply that an intimate correlation between miR-218-5p and PRKCE/MDR1 axis abnormal expression is a key determinant of gemcitabine tolerance, and suggest a novel miR-218-5p-based clinical intervention target for Gallbladder cancer patients. PMID:28492560
Resistin-associated vascular smooth muscle cell dysfunction and intimal hyperplasia are related to PKCepsilon-dependent Nox activation and ROS generation PMID:27573736
PKCepsilon is a key upstream mediator in resistin-induced inflammation that may interact synergistically with TLR4 to promote NF-kB activation in macrophages. PMID:28286252
protein kinase C-epsilon regulation, phospholipase D1 protects retinal pigment epithelium cells from lipopolysaccharide-induced damage PMID:27793751
we demonstrate that PKCepsilon cooperates with the loss of the tumor suppressor Pten for the development of prostate cancer in a mouse model. Mechanistic analysis revealed that PKCe overexpression and Pten loss individually and synergistically upregulate the production of the chemokine CXCL13, which involves the transcriptional activation of the CXCL13 gene PMID:28402859
These results indicate that PKC promotes synaptogenesis by activating PSD-95 phosphorylation directly through JNK1 and calcium/calmodulin-dependent kinase II and also by inducing expression of PSD-95 and synaptophysin. PMID:27330081
epsilonPKC phosphorylation and its coevolution with ALDH2 play an important role in the regulation and protection of ALDH2 enzyme activity. PMID:28056995
PKCepsilon down-regulation suppresses sorting and the cancer stem-like phenotype of renal cell carcinoma 769P side population cells through the regulation of ABCB1 transporter and the PI3K/Akt, Stat3 and MAPK/ERK pathways that are dependent on the phosphorylation effects. PMID:27037060
PRKCE plays two major roles in cardiac muscle cells: it takes part in regulating cardiac muscle contraction via targeting the sarcomeric proteins, as well as it modulates cardiac cell energy production and metabolism by targeting cardiac mitochondria. (Review) PMID:27312950
PKCepsilon is a key factor for driving the formation of bone metastasis by prostate cancer cells and is a potential therapeutic target for advanced stages of the disease. PMID:26023164
Depletion of PKCepsilon not only enhanced HGF-induced phosphorylation of JNK and paxillin (Ser178) but also prevented c-Met degradation. PMID:25778903
miR-34a regulates blood-tumor barrier (BTB) function by targeting PKCepsilon; after phosphorylation, PKCepsilon is activated and contributes to regulation of the expression of tight junction-related proteins, ultimately altering BTB permeability. PMID:25788289
PKCepsilon-CREB-Nrf2 signalling induces HO-1 in the vascular endothelium and enhances resistance to inflammation and apoptosis. PMID:25883219
PKC-delta activates p38/MAPK, responsible for the inhibition of MMP-2 and -9 secretion, PKC-epsilon activates a pathway made of ERK1/2, mTOR and S6K responsible for the inhibition of NHE1 activity and cell migration. PMID:25372487
HepaCAM may prevent the translocation of PKCepsilon from cytosolic to particulate fractions, resulting in the inhibition of 786-0 cell proliferation. PMID:24515280
Knockdown of PKCepsilon expression inhibited proliferation, induced apoptosis and decreased invasiveness of human glioma cell lines, and suppressed the growth of U87 cell-derived tumors in nude mice. PKCepsilon physically interacts with Stat3. PMID:24888992
PKCepsilon-mediated regulation has a role in protection from loss of chromosome integrity in cells failing to resolve catenation in G2 PMID:25483024
Data suggest that interleukin-32alpha (IL-32alpha) associates with leukemia zinc finger (PLZF) and protein kinase c epsilon (PKCvarepsilon), and then inhibits PLZF sumoylation, resulting in suppression of the transcriptional activity of PLZF. PMID:25178676
IL-32alpha inhibits BCL6 SUMOylation by activating PKCepsilon, resulting in the modulation of BCL6 target genes and cellular functions of BCL6. PMID:25245533
Suggest distinct role of PKCepsilon in controlling cell fate and immune response of monocyte subsets. PMID:25322815
MicroRNA-146a controls Th1-cell differentiation of human CD4-positive T-lymphocytes by targeting PKCepsilon. PMID:25308712
PKC epsilon regulates the interaction between Cx43 and Kir6.1 in the cardiomyocyte mitochondria and this interaction prevents hypoxia induced cell death. PMID:24815185
PKCepsilon activation may have therapeutic efficacy for AD by reducing neurotoxic Abeta accumulation as well as having direct anti-apoptotic and synaptogenic effects PMID:24848988
RNAi silencing of Sp1 and STAT1 in breast cancer cells reduced PKC mRNA and protein expression, as well as PRKCE promoter activity. PMID:24825907
a novel mechanism by which a PKC-Rab5a-Rac1 axis regulates cytoskeleton remodeling and T-cell migration, both of which are central for the adaptive immune response. PMID:24872409
VIP regulates CFTR membrane expression and function in Calu-3 cells by increasing its interaction with NHERF1 and P-ERM in a VPAC1- and PKCepsilon-dependent manner. PMID:24788249
PKCepsilon activation in late passage NP cells may represent a molecular basis for aggrecan availability, as part of an PKCepsilon/ERK/CREB/AP-1-dependent transcriptional program that includes upregulation of both chondrogenic genes and microRNAs PMID:24312401
our results identify a novel regulatory pathway involving the interplay between ZO-1, alpha5-integrin and PKCepsilon in the late stages of mammalian cell division. PMID:23967087
High PRKCE expression is associated with papillary thyroid tumor development. PMID:23457043
Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C epsilon-mediated zona occludens-1 phosphorylation at threonine 770/772. PMID:24338688
activated in compressive load-cultured nucleus pulposus cells PMID:24166186
MiR-143 specifically targets the 3'-UTR of PRKCE and regulates its expression. PMID:24070896
Data indicate that small molecules that block the oncogenic addiction to PKCepsilon signaling by promoting ATF2 nuclear export, resulting in mitochondrial membrane leakage and melanoma cell death. PMID:23589174
PKC epsilon plays central role in mediating HGF-induced fluctuant ERK-paxillin signaling, probably via the regulation of endosomal degradation of c-Met for the migration of HepG2. PMID:23524339
This study identifies PKCzeta as a novel key upstream regulator of BA-regulated SHP function, revealing the role of Thr-55 phosphorylation in epigenomic regulation of liver metabolism. PMID:23824184
similar to Ser-428/431 (in LKB1(L)), Ser-399 (in LKB1(S)) is a PKCzeta-dependent phosphorylation site essential for nucleocytoplasmic export of LKB1(S) and consequent AMPK activation. PMID:23612973
Upregulation of PKCeta contributes to breast cancer cell growth and targeting either PKCepsilon or PDK1 triggers PKCeta downregulation PMID:23562764
Phosphorylation of serine 779 in fibroblast growth factor receptor 1 and 2 by protein kinase C(epsilon) regulates Ras/mitogen-activated protein kinase signaling and neuronal differentiation. PMID:23564461
Ciliated cell detachment is associated with PKCepsilon autodownregulation in bronchial epithelium. PMID:22712879
Studies indicate that protein kinase C isoform epsilon (PKCepsilon) is differentially regulated in erythrocyte and megakaryocyte progenitors, suggesting an addressing role toward maturation of either lineage. PMID:23266964
MiR-31 directly targets PRKCE and thereby suppresses NF-kappaB activity and induces apoptosis and sensitivity to anti-cancer treatments via down-regulation of BCL2 expression. PMID:23364795
data suggest that platelet generations produced before the acute event retain PKCepsilon-mRNA that is not down-regulated during terminal megakaryocyte differentiation. PMID:23071564
Report metabolic cooperation between the EGFR/PKC-epsilon/NF-kappaB pathway in PKM2 upregulation and tumorigenesis. PMID:23123196