PEX19 Recombinant Monoclonal Antibody

Code: CSB-RA188883A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

P40855

Target Names

PEX19

Alternative Names

33 kDa housekeeping protein antibody; D1S2223E antibody; HK33 antibody; Housekeeping gene 33kD antibody; OK/SW-cl.22 antibody; PBD12A antibody; Peroxin 19 antibody; Peroxin-19 antibody; Peroxisomal biogenesis factor 19 antibody; Peroxisomal farnesylated protein antibody; PEX19 antibody; PEX19_HUMAN antibody; PMP1 antibody; PMPI antibody; PXF antibody; PXMP1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PEX19 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA188883A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

PEX19 serves as a critical cytosolic chaperone and import receptor essential for peroxisomal membrane protein biogenesis. This farnesylated protein recognizes and binds newly synthesized peroxisomal membrane proteins in the cytoplasm, guiding them to the peroxisomal membrane for proper insertion. Mutations in PEX19 are associated with Zellweger syndrome spectrum disorders, making this target particularly relevant for researchers investigating peroxisome biogenesis disorders, metabolic disease mechanisms, and organelle assembly pathways.

This recombinant monoclonal antibody, clone 8C2, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthesized peptide derived from human PEX19 protein and purified by affinity chromatography, ensuring high specificity for your target.

Validation in immunohistochemistry demonstrates reliable performance in formalin-fixed, paraffin-embedded human tissues. Testing in human liver cancer tissue sections using a Leica Bond system with citrate buffer antigen retrieval and HRP-polymer detection produced clear staining at 1:100 dilution, with recommended working concentrations ranging from 1:50 to 1:200 depending on your specific tissue and detection system. The antibody is also validated for ELISA applications, providing flexibility across different experimental platforms.

Whether you are investigating peroxisome assembly mechanisms, characterizing patient-derived samples for peroxisomal disorders, or studying the broader landscape of organelle biogenesis, this PEX19 antibody delivers the specificity and batch consistency needed for confident, reproducible results in your research.

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Target Background

Function(From Uniprot)

Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.

Gene References into Functions

Thus, PEX19 and PEX3 peroxisome biogenesis factors provide an alternative posttranslational route for membrane insertion of the reticulon homology domain-containing proteins, implying that endoplasmic reticulum membrane shaping and peroxisome biogenesis may be coordinated. PMID:29396426
The results demonstrate an allosteric mechanism for the modulation of PEX19 function by farnesylation. PMID:28281558
that newly synthesized UBXD8 is post-translationally inserted into discrete ER subdomains by a mechanism requiring cytosolic PEX19 and membrane-integrated PEX3, proteins hitherto exclusively implicated in peroxisome biogenesis PMID:27295553
suggest a novel regulatory mechanism for peroxisome biogenesis through the interaction between Pex19p and PLA/AT-3 PMID:26018079
Thus within the cell, PEX3 is stabilized by PEX19 preventing PEX3 aggregation. PMID:25062251
PEX19 formed a complex with the peroxisomal tail anchored protein PEX26 in the cytosol and translocated it directly to peroxisomes by a TRC40-independent class I pathway. PMID:23460677
PEX3-PEX19 interaction is crucial for de novo formation of peroxisomes in peroxisome-deficient cells. PMID:22624858
The Pex19p peptide contains a characteristic motif, consisting of the leucine triad (Leu18, Leu21, Leu22), and Phe29, which are critical for the Pex3p binding and peroxisome biogenesis. PMID:21102411
The crystal structure of the cytosolic domain of PEX3 in complex with a PEX19-derived peptide. PEX3 adopts a novel fold that is best described as a large helical bundle. PMID:20554521
data indicate a divided N-terminal and C-terminal structural arrangement in Pex19p, which is reminiscent of a similar division in the Pex5p receptor, to allow separation of cargo-targeting signal recognition and additional functions. PMID:20531392
a considerable functional diversity of the proteins encoded by two PEX19 splice variants and thereby provide first experimental evidence for specific biological functions of the different predicted domains of the PEX19 protein. PMID:11883941
PEX19 binds and stabilizes newly synthesized PMPs in the cytosol, binds to multiple PMP targeting signals (mPTSs), interacts with the hydrophobic domains of PMP targeting signals, and is essential for PMP targeting and import. PMID:14709540
Interaction of PEX3 and PEX19 visualized by fluorescence resonance energy transfer (FRET). PMID:14713233
Pex19p has a role in assembly of PTS-receptor docking complexes PMID:14715663
Results suggest that PEX3 plays a selective, essential, and direct role in class I peroxisomal membrane protein import as a docking factor for PEX19. PMID:15007061
human Pex19p domain architecture and activity PMID:15252024
analysis of the PEX19-binding site of human adrenoleukodystrophy protein PMID:15781447
Pex19p translocates the membrane peroxins from the cytosol to peroxisomes in an ATP- and Pex3p-dependent manner and then shuttles back to the cytosol PMID:16280322
Pex19p binds to PMP70 co-translationally and keeps PMP70 in a proper conformation for the localization to peroxisome. PMID:16344115
Nonfarnesylated and farnesylated human Pex19p display a similar affinity towards a select set of peroxisomal membrane proteins. PMID:16791427
Data suggest that Pex19p probably functions as a chaperone for membrane proteins and transports them to peroxisomes by anchoring to Pex3p using residues 12-73 and 40-131. PMID:16895967
either one or two tryptophan residues of Pex3p (Trp-104 and Trp-224) are directly involved in binding to Pex19p. PMID:18174172
targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins. PMID:18782765
N-terminal domain of Pex14, Pex14(N), adopts a three-helical fold. Pex5 and Pex19 ligand helices bind competitively to the same surface in Pex14(N) albeit with opposite directionality. PMID:19197237
ALDRP interacts with PEX19 splice variants PEX19-delta-E2 and PEX19-delta-E8. PMID:11883941
LDRP (ABCD2) interacts with both farnesylated wild-type and farnesylation-deficient mutant PEX19. This interaction is mediated by amino acids 1-218 of ALDRP. PMID:10777694
MP70 (ABCD3) interacts with both farnesylated wild-type and farnesylation-deficient mutant PEX19. PMID:10777694
MP70 interacts with PEX19 splice variants PEX19-delta-E2 and PEX19p-delta-E8. PMID:11883941