PECAM1 Recombinant Monoclonal Antibody

Code: CSB-RA017767MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, FC

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Target Background

Product Details

Uniprot NO.

P16284

Target Names

PECAM1

Alternative Names

Adhesion molecule antibody; CD31 antibody; CD31 antigen antibody; CD31 EndoCAM antibody; EndoCAM antibody; FLJ34100 antibody; FLJ58394 antibody; GPIIA antibody; GPIIA' antibody; PECA1 antibody; PECA1_HUMAN antibody; Pecam 1 antibody; PECAM 1 CD31 EndoCAM antibody; PECAM antibody; PECAM-1 antibody; Pecam1 antibody; Platelet and endothelial cell adhesion molecule 1 antibody; Platelet endothelial cell adhesion molecule antibody; Platelet/endothelial cell adhesion molecule 1 antibody

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human PECAM1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG2a

Clone No.

9C9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:20-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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Western Blot
Positive WB detected in: THP-1 whole cell lysate, JK whole cell lysate
All lanes: PECAM1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 82-140 kDa
Observed band size: 140 kDa
Overlay Peak curve showing THP-1 cells stained with CSB-RA017767MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

PECAM1, also known as CD31, serves as a critical adhesion molecule expressed predominantly on endothelial cells, platelets, and various leukocyte populations. This transmembrane glycoprotein plays essential roles in angiogenesis, leukocyte transmigration across the endothelium, and maintaining vascular integrity. Its involvement in cell-cell interactions makes it an indispensable marker for vascular biology research, tumor microenvironment studies, and immunological investigations.

This recombinant monoclonal antibody (clone 9C9) offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports reliable data across longitudinal studies and multi-site collaborations. The affinity-purified IgG2a format ensures minimal background interference in sensitive detection systems.

Validation studies demonstrate robust performance across multiple applications. In Western blot analysis, the antibody detects PECAM1 at an observed molecular weight of 140 kDa in THP-1 monocytic and Jurkat cell lysates at dilutions of 1:1000–1:5000. The difference between predicted and observed band sizes reflects the extensive glycosylation characteristic of this cell surface adhesion molecule. Flow cytometry validation in THP-1 cells confirms specific detection with clear separation from isotype control, supporting applications in cell phenotyping and population analysis at dilutions of 1:20–1:200. The antibody demonstrates cross-species reactivity with both human and mouse samples, providing flexibility for translational research models.

Whether investigating endothelial dysfunction in cardiovascular disease, characterizing tumor vasculature, examining stem cell differentiation, or studying immune cell trafficking, this PECAM1 antibody delivers the specificity and consistency needed for meaningful experimental outcomes.

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Target Background

Function(From Uniprot)

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.; Does not protect against apoptosis.

Gene References into Functions

High CD31 expression is associated with Central Giant Cell Granuloma. PMID:30139237
TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID:29949812
High CD31 expression is associated with early-stage, but not in late-stage, laryngeal squamous cell carcinoma. PMID:29523110
Adenocarcinomas showed significantly higher staining scores of both VEGF and alphaSMA than squamous cell carcinomas did. In 42 cases of high CD31 score, five-year survival rate (87%) of patients with lung cancer showing mature tumor vessels was significantly better than that (69%) of patients with immature tumor vessels PMID:29970531
Differences in trafficking of CD31(+) cytotoxic T lymphocytes during acute influenza infection could modulate tolerance and contribute to a dampened adaptive immune response in neonates PMID:28355204
Cell adhesion assays on wildtype and mutant PECAM-1 further characterized the structural determinants in cell junction and communication. PMID:27958302
In primary hip OA, angiogenesis may be induced by a combined mechanism: hypoxia-related VEGF-dependent vasculogenesis and endothelial differentiation of the activated pluripotent cells, which are released from the hyperplastic synovial cells layer. An endothelial mesenchymal transition is assumed to be involved in the fibrotic process. PMID:27704157
upregulation of sVEGFR-1 with concomitant decline of PECAM-1 and sVEGFR-2 levels in preeclampsia compared to normotensive pregnancies, Irrespective of the HIV status PMID:28609170
Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD. PMID:27552332
PECAM-1 gene polymorphisms are associated with Kawasaki disease with and without coronary artery lesions in Chinese children. PMID:28512385
Sirt1 expression is associated with CD31 expression in endothelial progenitor cells from patients with chronic obstructive pulmonary disease. PMID:27784320
we found a significant cumulative contribution of the genetic heterogeneity of glycoproteins Ia and IIIa and platelet-endothelial cell adhesion molecule-1 and P-Selectin genes in the risk of recurrent IVF-ET failures. The coexistence of these SNPs was associated with increased IVF-ET failure risk and the more polymorphic alleles or genotypes were present the higher the risk of IVF-ET failure, especially for younger women PMID:28388872
Dimer conformation of soluble PECAM-1 PMID:27270333
RrgA, binds both polymeric immunoglobulin receptor (pIgR) and PECAM-1, whereas the choline binding protein PspC binds, but to a lower extent, only pIgR PMID:28515075
this study shows a significant role for CD31 during T cell development PMID:28159903
Cells in high glucose for 7 days showed a significant decrease in mRNA expression of CD31 and VE-cadherin, and a significant increase in that of alpha-SMA and collagen I. PMID:28347704
Patients who had optic neuritis that progressed to multiple sclerosis had a decrease in serum PECAM-1 levels. PMID:27806869
Platelet endothelial cell adhesion molecule (PECAM-1) is expressed in endothelial cells (ECs), platelets, and leukocytes, regulating the interaction between those cells. PMID:27079772
Data indicate no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with fetal growth restriction (FGR). PMID:28358707
these data suggest that a sialic acid-containing glycan emanating from Asn-25 reinforces dynamic endothelial cell-cell interactions by stabilizing the PECAM-1 homophilic binding interface. PMID:27793989
Decreased FoxP3 expression in CD31(+) Tr cells. PMID:27997991
These results suggested that PECAM-1 could mediate platelet adhesion to endothelial cells under shear stress. Platelets binding to endothelial cells interfered with endothelial cell mechanotransduction through PECAM-1, affecting endothelial cell inflammatory responses towards pathological shear flow. PMID:28013181
Immunohistochemical expression of CD31 and vascular endothelial growth factor (VEGF) were assessed in parallel. PMID:27270504
CD31 is expressed in mycosis fungoides (MF) skin biopsies, which provides new evidence for the role of angiogenesis in the progression of MF PMID:27630298
The Leu125Val polymorphism of PECAM-1 and the level of soluble PECAM-1 are not associated with diabetic nephropathy in Caucasians with type 2 diabetes mellitus. PMID:28116228
We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID:26748901
The PECAM-1 functions as an adhesive stress-response protein to both maintain endothelial cell junctional integrity and speed restoration of the vascular permeability barrier following inflammatory or thrombotic challenge. PMID:27055047
These studies indicate a role for PECAM-1 in enhancing the inhibitory functions of TGF-beta in T cells PMID:26956486
PECAM1 plays an important role in the formation of tight junction complex. PMID:26607202
The most significant associations were detected for PECAM1*V/V + DDAH1*C (OR = 4.17 CI 1.56-11.15 Pperm = 0.005) PMID:26662939
soluble CD38 (sCD38) in seminal plasma increases the capacitation of sperm via specific interactions between sCD38 and the CD31 on the sperm. PMID:26407101
expression levels of CD31/ PECAM1 are deregulated in human glioblastoma multiforme tissue specimens; correlation among CD31/PECAM1 and HIF-1alpha and N-cadherin and ADAM-10, two other markers of aggressiveness in the same tumors PMID:26376118
Radiation-induced stress conditions induce a transient accumulation of granulocytes within the liver by down-regulation/absence of PECAM-1. PMID:26177067
Suggest that CD31 expression correlates with prognosis in gastrointestinal stromal tumors. PMID:26078569
PECAM-1 125C/G polymorphism is associated with deep vein thrombosis. PMID:25846278
Heterogeneity was found in the endothelial cells: their shape, the expression of adhesion molecules(ICAM-1, VCAM-1, and PECAM ), and the adhesion of lymphocytes and monocytes to them changed during the progression of the atherosclerotic process. PMID:26841644
PECAM1+ melanoma cells form vascular channels PMID:25335460
Low shear stress can induce inflammatory response via PECAM-1/PARP-1/HMGB1 pathway. PMID:25793984
Nck promoted oxidative stress-induced activation of NF-kappaB by coupling the tyrosine phosphorylation of PECAM-1 (platelet endothelial cell adhesion molecule-1) to the activation of p21-activated kinase PMID:25714462
PECAM-1 has a role in mediating the profibrotic and prometastasic environment caused by ethanol in endothelial cells PMID:24734240
Report correlation between mast cell tryptase and CD31 expression in odontogenic tumors. PMID:26247531
there are associations between various PECAM-1 polymorphisms in rheumatoid arthritis and systemic lupus erythematosus patients, and PECAM-1 polymorphisms in SLE are protective against atherosclerotic complications PMID:25201689
preeclampsia does not significantly affect vascular growth or the expression of endothelial junction proteins in human placentas PMID:25362142
C-CD31 have impaired angiogenic potential and the number of circulating CD31(+) cells were correlated with cardiovascular disease risk PMID:25267411
demonstrate a functional link between HO-1 gene expression and PECAM-1 in endothelial cells, which might play a critical role in the regulation of inflammation PMID:24500083
The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. PMID:25884075
High CD31 expression associated significantly with better survival and VEGFR3 had no association with survival. Both higher tumor grade and stage were associated with a decreased survival time PMID:25667475
Suggest that cell confluence and the type of flow are critical independent factors in the induction of TF and PECAM-1 phosphorylation in endothelial cells exposed to disturbed pulsatile flow and chemical stimuli. PMID:24342062
The finding that the adhesive properties of PECAM-1 are regulatable suggests novel approaches for controlling endothelial cell migration and barrier function in a variety of vascular permeability disorders. PMID:24936065
for the cytological diagnosis of angiosarcomas, ERG and CD31 are more sensitive vascular markers than CD34 PMID:25352641

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.; [Isoform Long]: Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell junction.; [Isoform Delta15]: Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

Tissue Specificity

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predom

Database Links

HGNC: 8823

UNIGENE: Hs.376675

KEGG: hsa:5175

OMIM: 173445

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