P4HTM Recombinant Monoclonal Antibody

Code: CSB-RA268805A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9NXG6

Target Names

P4HTM

Alternative Names

EGLN4 antibody; HIF PH4 antibody; HIF Prolyl Hydroxylase 4 antibody; HIF prolyl hydroxylase PH 4 antibody; HIF prolyl hydroxylase PH4 antibody; HIF-PH4 antibody; HIF-prolyl hydroxylase 4 antibody; HIFPH4 antibody; HPH 4 antibody; HPH-4 antibody; Hypoxia inducible factor prolyl 4 hydroxylase antibody; Hypoxia-inducible factor prolyl hydroxylase 4 antibody; P4H with transmembrane domain antibody; P4H-TM antibody; P4htm antibody; P4HTM_HUMAN antibody; PH 4 antibody; PH4 antibody; PHD 4 antibody; Proline 4 hydroxylase antibody; prolyl 4-hydroxylase antibody; Transmembrane prolyl 4-hydroxylase antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human P4HTM protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1B12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: U-251MG whole cell lysate(30µg), HT-29 whole cell lysate(30µg), A549 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), THP-1 whole cell lysate(30µg)
All lanes:HIF PH4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa
Exposure time：1min
IHC image of CSB-RA268805A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing PC-3 cells stained with CSB-RA268805A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

P4HTM, also known as HIF prolyl hydroxylase 4, represents a distinctive member of the prolyl hydroxylase family that regulates hypoxia-inducible factor stability. Unlike its cytoplasmic counterparts, P4HTM contains a transmembrane domain that localizes it to the endoplasmic reticulum, positioning it uniquely within cellular oxygen-sensing pathways. This enzyme plays important roles in cardiovascular research, where understanding hypoxia response mechanisms remains central to investigating ischemic conditions and tissue adaptation.

This recombinant monoclonal antibody, clone 1B12, offers researchers the reproducibility essential for longitudinal studies and multi-site collaborations. Developed through recombinant technology using a synthesized peptide from human P4HTM protein, it provides sequence-defined specificity with consistent performance across production lots, eliminating the variability that can complicate experimental interpretation.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis confirms detection in diverse human cell lines including U-251MG glioblastoma, HT-29 colorectal adenocarcinoma, A549 lung carcinoma, MCF-7 breast cancer, HepG2 hepatocellular carcinoma, and THP-1 monocytic cells, with an observed band at approximately 55 kDa. The slight difference from the predicted 57 kDa molecular weight likely reflects post-translational processing or conformational factors common to membrane-associated proteins. Immunohistochemistry validation in human placenta tissue using citrate buffer antigen retrieval demonstrates clear staining patterns, while flow cytometry analysis in PC-3 prostate cancer cells shows distinct positive population shifts compared to isotype controls.

Whether investigating oxygen-sensing mechanisms in cardiovascular disease models or characterizing P4HTM expression across tissue types, this antibody provides the technical foundation for reliable, reproducible results in your hypoxia-related research.

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Target Background

Function(From Uniprot)

Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Gene References into Functions

The results suggest that SESN2 increases degradation of HIF-1A via AMPK-PHD regulation that contributes to inhibition of in vitro and in vivo tumorigenesis. PMID:27840318
This review will expand our knowledge of biology of HIFs, PHDs, PHD inhibitors, and bone regeneration, and it may also aid the design of novel therapies for accelerating bone repair and regeneration or inhibiting bone tumours. [review] PMID:24895555
Results suggest that increased expression of prolyl hydroxylase (PH) might play an role in the physiology of uterine leiomyoma during the menstrual cycle. PMID:23241241
Data suggest that PH-4 is a novel hypoxia-inducible factor-prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. PMID:12163023
the longest and shortest isoenzymes have major transcripts encoding inactive polypeptides, which suggest novel regulation by alternative splicing. PMID:12788921
crystal structure of the peptide-substrate-binding domain PMID:15456751
This review summarizes recent progress in elucidating the molecular mechanisms of hypoxia-inducible factor (HIF)-1 activation, focusing on the role of oxygen-dependent asparaginyl hydroxylase in hypoxia signal transduction. PMID:16154531

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Tissue Specificity

Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.

Database Links

HGNC: 28858

UNIGENE: Hs.654944

KEGG: hsa:54681

STRING: 9606.ENSP00000341422

OMIM: 614584

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