NFKBIE Recombinant Monoclonal Antibody

Code: CSB-RA288692A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O00221

Target Names

NFKBIE

Alternative Names

NF-kappa-B inhibitor epsilon (NF-kappa-BIE) (I-kappa-B-epsilon) (IkB-E) (IkB-epsilon) (IkappaBepsilon), NFKBIE, IKBE

Species Reactivity

Human

Immunogen

A synthesized peptide from human NFKBIE protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5F3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: THP-1 whole cell lysate(30µg), K562 whole cell lysate(30µg)
All lanes: IkB epsilon antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time：1min40s
IHC image of CSB-RA288692A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA288692A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A431 cell with CSB-RA288692A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Raji cells stained with CSB-RA288692A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

NFKBIE, also known as IκB-epsilon, serves as a critical regulator of NF-κB signaling by sequestering NF-κB transcription factors in the cytoplasm and preventing their nuclear translocation. This inhibitory function makes NFKBIE an important target for researchers investigating inflammatory responses, immune regulation, and cancer biology, where dysregulated NF-κB activity contributes to disease progression.

This recombinant monoclonal antibody, generated from clone 5F3, offers the reproducibility and consistency that demanding research applications require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in long-term studies.

Validation data demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis detects NFKBIE at the expected 53 kDa molecular weight in human THP-1 and K562 cell lysates, confirming specificity in both monocytic and erythroleukemic cell models commonly used in NF-κB pathway research. Immunohistochemistry staining has been successfully performed on paraffin-embedded human lung cancer and thyroid tissue sections using citrate buffer antigen retrieval, providing options for tissue-based studies. Immunofluorescence analysis in A431 cells reveals subcellular localization patterns, while flow cytometry validation in Raji cells demonstrates clear positive signal separation from isotype controls, supporting intracellular staining applications.

The unconjugated format allows flexibility in secondary antibody selection across detection systems. Whether you are examining NF-κB pathway dynamics in inflammation models, characterizing IκB family member expression in tumor samples, or investigating immune cell signaling, this antibody provides a reliable tool for interrogating NFKBIE biology in human samples.

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Target Background

Function(From Uniprot)

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Gene References into Functions

identify NFKBIE aberrations as a common genetic event across B-cell malignancies and highlight NFKBIE deletions as a novel poor-prognostic marker in PMBL. PMID:27670424
We suggest that the impairment of NFKBIE gene function can reduce the uptake of methotrexate into cells, suggesting that the gene is an important factor for rheumatoid arthritis outcome PMID:26587663
A NFKBIE polymorphism was associated with increased risk of pneumococcal meningitis. PMID:26870821
results show that Gal-1 acts by inhibiting the stimulation of the LPS-induced IkappaBzeta expression, an NF-kappaB regulator involved in IL-6 gene transcription. PMID:26226212
Newly identified alterations included recurrent promoter mutations of NFKBIE, encoding NF-kappaB inhibitor varepsilon (IkappaBvarepsilon), in 14.5% of samples of desmoplastic melanoma PMID:26343386
Vitamin C forestalls cigarette smoke induced NF-kappaB activation in alveolar epithelial cells. PMID:23615073
we identified two gene loci associated with rheumatoid arthritis susceptibility- NFKBIE and RTKN2 PMID:23028356
Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon PMID:16769727
An NFKBIE SNP associated with susceptibility to pneumococcal disease but not pneumococcal empyema. PMID:17463416
Expression levels of GGT1 and possibly NFKBIE might be useful as biomarkers of genetic susceptibility to arsenite. PMID:17976673
Genomic mutation of the NFKBIE gene in primary Hodgkin/ Reed Sternberg cells PMID:14595753